Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

DNA extraction from paraffin material protocol in order to analyse microssatellites in uterine leiomyoma

Full text
Author(s):
Ericson Martins Nascimento [1] ; Marilda Osti Spinelli [2] ; Consuelo Junqueira Rodrigues [3] ; Nilo Bozzini [4]
Total Authors: 4
Affiliation:
[1] Universidade de São Paulo. Faculdade de Medicina. Departamento de Cirurgia
[2] Universidade de São Paulo. Faculdade de Medicina. Departamento de Cirurgia
[3] Universidade de São Paulo. Faculdade de Medicina. Departamento de Cirurgia
[4] Hospital das Clínicas de São Paulo. Serviço de Ginecologia e Obstetrícia
Total Affiliations: 4
Document type: Journal article
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial; v. 39, n. 3, p. 253-255, 2003-09-00.
Abstract

The standardization of the protocol of the DNA extraction from paraffin material in order to analyze the instability of microsatellites in genes related to the uterine leiomyoma, using 30 to 40mg of tissue removed from paraffin blocks is presented. Desparaffin throught xilol baths to 65ºC, rehydrated with solutions of decreasing concentrations of ETOH and deionisation water. The extraction of DNA was obtained through the stages of cellular lysis, with Promega solution, digestion of proteins by Proteinase K, precipitation proteins with Promega solution and precipitation of DNA with ETOH 70% colded and ressuspended with Promega solution. This protocol results in a viable DNA to be use in the Polimerase Chain Reaction (PCR). (AU)