Alkaline phosphatase activity in plasma and liver of rats submitted to chronic exposure to fluoride

Author(s)

Mileni da Silva Fernandes ^[1] ; Flávia Godoy Iano ^[2] ; Vivian Rocia ^[1] ; Marcela Mitsuko Yanai ^[1] ; Aline de Lima Leite ^[1] ; Tatiana Almeida Furlani ^[1] ; Marília Afonso Rabelo Buzalaf ^[7] ; Rodrigo Cardoso de Oliveira ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Odontol Bauru, Dept Ciencias Biol, BR-17012901 Bauru, SP - Brazil

^[2] Universidade de São Paulo. Faculdade de Odontologia de Bauru - Brasil

^[3] Universidade de São Paulo. Faculdade de Odontologia de Bauru - Brasil

• Document type: Journal article
• Source: Brazilian Archives of Biology and Technology; v. 54, n. 6, p. 1187-1192, 2011-12-00.

Abstract

The aim of this study was to compare the effect of fluoride (F) on alkaline phosphatase activity in the liver and plasma of the rats. Four groups of male Wistar rats (n=6), which received drinking water containing 5, 15 or 50 ppm F or deionized water (control) throughout the experiment were included in the study. The animals were euthanized and had their tissues and blood plasma collected for the analysis of fluoride and alkaline phosphatase. There was an increase in F concentration in most tissues in the animals treated with higher F concentrations, except for the heart. The alkaline phosphatase assay showed an increase in the activity in the liver and blood plasma of the animals treated with fluoride concentrations of 15 and 50 ppm (p<0.05). This study suggested that F at a concentration of 50 ppm in drinking water promotes increased the activity of alkaline phosphatase in the liver and blood plasma. (AU)

• FAPESP's process: 06/06430-3 - Analysis of the influence of fluoride in bone repair in dental alveoli in rats
• Grantee: Rodrigo Cardoso de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 07/00494-2 - Fluoride influence on alveolar bone repair in rats: histological and immunohistochemical evaluation
• Grantee: Marcela Mitsuko Yanai
• Support Opportunities: Scholarships in Brazil - Scientific Initiation