Interleukin-8-251T > A, Interleukin-1 alpha-889C > T and Apolipoprotein E polymorphisms in Alzheimer's disease

Author(s):Show less -

Vendramini, Alex Augusto ^[1] ; de Labio, Roger Willian ; Rasmussen, Lucas Trevizani ^[2] ; dos Reis, Nathali Mattiuzo ^[2] ; Minett, Thais ^[3] ; Ferreira Bertolucci, Paulo Henrique ^[3] ; de Souza Pinhel, Marcela Augusta ^[4] ; Silva Souza, Doroteia Rossi ^[4] ; Mazzotti, Diego Robles ^[2] ; Cardoso Smith, Marilia de Arruda ^[2] ; Marques Payao, Spencer Luiz ^{[1, 5]}

Affiliation

^[1] Univ Sagrado Coracao, Bauru, SP - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Morfol, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Escola Paulista Med, Disciplina Neurol, Sao Paulo, SP - Brazil

^[4] Fac Med Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP - Brazil

^[5] Fac Med Marilia, Hemoctr, Genet Lab, Disciplina Genet, BR-17519050 Marilia, SP - Brazil

• Document type: Journal article
• Source: GENETICS AND MOLECULAR BIOLOGY; v. 34, n. 1, p. 1-5, 2011.
• Web of Science Citations: 9

Abstract

An inflammatory process has been involved in numerous neurodegenerative disorders such as Parkinson's disease, stroke and Alzheimer's disease (AD). In AD, the inflammatory response is mainly located in the vicinity of amyloid plaques. Cytokines, such as interleukin-8 (IL-8) and interleukin-1 alpha (IL-1 alpha), have been clearly involved in this inflammatory process. Polymorphisms of several interleukin genes have been correlated to the risk of developing AD. The present study investigated the association of AD with polymorphisms IL-8-251T > A (rs4073) and IL-1 alpha-889C > T (rs1800587) and the interactive effect of both, adjusted by the Apolipoprotein E genotype. 199 blood samples from patients with AD, 146 healthy elderly controls and 95 healthy young controls were obtained. DNA samples were isolated from blood cells, and the PCR-RFLP method was used for genotyping. The genotype distributions of polymorphisms IL-8, IL-1 alpha and APOE were as expected under Hardy-Weinberg equilibrium. The allele frequencies did not differ significantly among the three groups tested. As expected, the APOE4 allele was strongly associated with AD (p < 0.001). No association of AD with either the IL-1 alpha or the IL-8 polymorphism was observed, nor was any interactive effect between both polymorphisms. These results confirm previous studies in other populations, in which polymorphisms IL-8-251T > A and IL-1 alpha-889C > T were not found to be risk factors for AD. (AU)

• FAPESP's process: 04/15273-3 - Characterization of ribosomal genes and of apolipoprotein AV and interleukin 6, 8 and 1st (alpha) polymorphisms in patients with Alzheimer's Disease
• Grantee: Spencer Luiz Marques Payão
• Support Opportunities: Regular Research Grants