Ribosomal gene expression of B-amyloid precursor protein, of presenilin and cytokines in brain and blood samples of Alzheimer's disease patients by Real Time PCR technique

• Grant number: 09/15857-9
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2010
• End date: October 31, 2012
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Spencer Luiz Marques Payão
• Grantee: Spencer Luiz Marques Payão
• Host Institution: Faculdade de Medicina de Marília (FAMEMA). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). Marília , SP, Brazil

Abstract

Alzheimer's disease is a neurodegenerative, irreversible and progressive disorder that is characterized by cognition's decline, loss of memory and mental confusion. The etiology of this disease is complex and genetically heterogeneous. There is evidence that at least four different genetic loci can confer inherited susceptibility to Alzheimer's disease. Mutations in the amyloid precursor gene (APP) located in chromosome 21q21.1, in the chromosome 14q24.3, gene which encodes presenilin 1 (PS1) and in the Presenilin-2 gene (PS2) located in chromosome 1q42.1 can be associated with Alzheimer's disease. The allele e4 for apolipoprotein E gene (APOE) located in 19q13.2, associated with an increased risk for the development of AD. Our group have shown a decrease of ribosomal gene activity in Alzheimer's disease using blood samples. The present project will use brain samples and blood samples of Alzheimer's disease patients and controls and it has the follow objectives: to characterize the gene expression of ribosomal genes (28S and 18S), ABPP, PS1, PS2, interleukin 1beta e 8 by Real Time PCR; to verify the polymorphisms of the promoter 1beta e 8 genes. This is the original study in the literature to compare the expression of these genes simultaneously in Alzheimer's disease and can propose the new way for understanding this important disease. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RASMUSSEN, LUCAS; DE LABIO, ROGER W.; VIANI, GUSTAVO A.; CHEN, ELIZABETH; VILLARES, JOAO; BERTOLUCCI, PAULO-HENRIQUE; MINETT, THAIS S.; TURECKI, GUSTAVO; CECYRE, DANIELLE; DRIGO, SANDRA A.; et al. Differential Expression of Ribosomal Genes in Brain and Blood of Alzheimer's Disease Patients. Current Alzheimer Research, v. 12, n. 10, p. 984-989, 2015. (09/10777-7, 09/15857-9)

DA COSTA, ISABELA BAZZO; DE LABIO, ROGER WILLIAN; RASMUSSEN, LUCAS TREVIZANI; VIANI, GUSTAVO ARRUDA; CHEN, ELIZABETH; VILLARES, JOAO; TURECKI, GUSTAVO; CARDOSO SMITH, MARILIA DE ARRUDA; PAYAO, SPENCER L. M.. Change in INSR, APBA2 and IDE Gene Expressions in Brains of Alzheimer's Disease Patients. Current Alzheimer Research, v. 14, n. 7, p. 760-765, 2017. (09/10777-7, 09/15857-9)

MARQUES PAYAO, SPENCER LUIZ; GONCALVES, GISELA MORAES; DE LABIO, ROGER WILLIAM; HORIGUCHI, LIE; MIZUMOTO, IGOR; RASMUSSEN, LUCAS TREVIZANI; DE SOUZA PINHEL, MARCELA AUGUSTA; SILVA SOUZA, DOROTEIA ROSSI; BECHARA, MARCELO DIB; CHEN, ELIZABETH; et al. Association of interleukin 1 beta polymorphisms and haplotypes with Alzheimer's disease. Journal of Neuroimmunology, v. 247, n. 1-2, p. 59-62, JUN 15 2012. (04/15273-3, 09/15857-9, 06/07240-3)

FUKASAWA, JOSIANNE T.; DE LABIO, ROGER W.; RASMUSSEN, LUCAS T.; DE OLIVEIRA, LUCIENI C.; CHEN, ELIZABETH; VILLARES, JOAO; TURECK, GUSTAVO; SMITH, MARILIA DE ARRUDA C.; PAYAO, SPENCER L. M.. CDK5 and MAPT Gene Expression in Alzheimer's Disease Brain Samples. Current Alzheimer Research, v. 15, n. 2, p. 182-186, 2018. (09/15857-9, 15/24699-9)

RASMUSSEN, LUCAS; DELABIO, ROGER; HORIGUCHI, LIE; MIZUMOTO, IGOR; TERAZAKI, CARLOS-RENATO; MAZZOTTI, DIEGO; BERTOLUCCI, PAULO-HENRIQUE; PINHEL, MARCELA-AUGUSTA; SOUZA, DOROTEIA; KRIEGER, HENRIQUE; et al. Association Between Interleukin 6 Gene Haplotype and Alzheimer's Disease: A Brazilian Case-Control Study. JOURNAL OF ALZHEIMER'S DISEASE, v. 36, n. 4, p. 733-738, 2013. (04/15273-3, 09/15857-9, 06/07240-3)

DELABIO, ROGER; RASMUSSEN, LUCAS; MIZUMOTO, IGOR; VIANI, GUSTAVO-ARRUDA; CHEN, ELIZABETH; VILLARES, JOAO; COSTA, ISABELA-BAZZO; TURECKI, GUSTAVO; LINDE, SANDRA APARECIDO; SMITH, MARILIA CARDOSO; et al. PSEN1 and PSEN2 Gene Expression in Alzheimer's Disease Brain: A New Approach. JOURNAL OF ALZHEIMER'S DISEASE, v. 42, n. 3, p. 757-760, 2014. (09/10777-7, 09/15857-9)