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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Modulation of cell proliferation, survival and gene expression by RAGE and TLR signaling in cells of the innate and adaptive immune response: role of p38 MAPK and NF-KB

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Author(s):
de Medeiros, Marcell Costa [1] ; Tfaile Frasnelli, Sabrina Cruz [1] ; Bastos, Alliny de Souza [1] ; Perez Orrico, Silvana Regina [1] ; Rossa Junior, Carlos [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista, UNESP, Sch Dent, Dept Diag & Surg, Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Applied Oral Science; v. 22, n. 3, p. 185-193, MAY-JUN 2014.
Web of Science Citations: 31
Abstract

Objective: The aim of this study was to evaluate a possible synergism between AGE-RAGE and TLR4 signaling and the role of p38 MAPK and NF-kB signaling pathways on the modulation of the expression of inflammatory cytokines and proliferation of cells from the innate and adaptive immune response. Material and Methods: T lymphocyte (JM) and monocyte (U937) cell lines were stimulated with LPS and AGE-BSA independently and associated, both in the presence and absence of p38 MAPK and NF-kB inhibitors. Proliferation was assessed by direct counting and viability was assessed by a biochemical assay of mitochondrial function. Cytokine gene expression for RAGe, CCL3, CCR5, IL-6 and TNF-α was studied by RT-PCR and RT-qPCR. Results: RAGE mRNA expression was detected in both cell lines. LPS and AGE-BSA did not influence cell proliferation and viability of either cell line up to 72 hours. LPS and LPS associated with AGE induced expression of IL-6 and TNF-α in monocytes and T cells, respectively. Conclusions: There is no synergistic effect between RAGE and TLR signaling on the expression of IL-6, TNF-α , RAGE, CCR5 and CCL3 by monocytes and lymphocytes. Activation of RAGE associated or not with TLR signaling also had no effect on cell proliferation and survival of these cell types. (AU)

FAPESP's process: 10/06589-8 - Effect of lipid oxidation associated or not with advanced glycation end-products on intracellular signaling pathways and on the expression of inflammatory mediators
Grantee:Carlos Rossa Junior
Support Opportunities: Regular Research Grants