| Grant number: | 17/02998-0 |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| Effective date (Start): | June 01, 2017 |
| Effective date (End): | August 31, 2020 |
| Field of knowledge: | Biological Sciences - Immunology - Applied Immunology |
| Cooperation agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal researcher: | Maria Cristina Roque Antunes Barreira |
| Grantee: | Rafael Ricci de Azevedo |
| Home Institution: | Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
| Associated scholarship(s): | 18/16374-0 - Transcriptome analysis of Toxoplasma gondii-infected human neutrophils: roles of MIC1 and MIC4, BE.EP.PD |
Abstract Interest in studying neutrophils has been increased in recent years among many research groups, which look for characterizing new functions and identifying the role exerted by neutrophils in different pathologies. Numerous obligatory or facultative intracellular pathogens can escape from neutrophil responses and thus live and reproduce in the host organism. Some pathogens modulate neutrophils functions to be successful in the infection process, regulating the activation of signaling pathways and controlling host cell survival. The interactions between neutrophils and the pathogens Toxoplasma gondii or Paracoccidioides brasiliensis are poorly studied, concerning the intracellular signaling mechanisms. Indeed, it is known that both pathogens activate myeloid cells through the binding to Toll-like receptors (TLRs). Our research group studies the lectins' interaction with glycoproteins on the surface of mammalian cells, including TLRs. These studies led us to identify and characterize some pathogens' lectins, such as TgMIC1 and TgMIC4, from T. gondii, and Paracoccina (PCN) from P. brasiliensis. These lectins bind to TLRs' N-glycans on myeloid cells, eliciting appropriate responses for influencing the host immunity. Since neutrophils are the first cells migrating into infected sites and intracellular pathogens can subvert the neutrophils' microbicide mechanisms, it is crucial to understand in detail the interaction established between intracellular pathogens and neutrophils. Therefore, the aim of this work is to evaluate the effect of the pathogens T. gondii and P. brasiliensis, and their lectins, on the activation of neutrophil intracellular signaling pathways, triggered by the recognition of glycans N-linked to TLRs. Then, human neutrophils will be stimulated with the mentioned lectins or infected with the focused pathogens and assessed by western blot for the activation of signaling pathways, such as MAPK - P38 and ERK1 / 2, mTOR, AKT, PI3K, JNK, TAK1. We will used the same approach in assays with transformed pathogens, which are knocked out, deficient or presenting superexpression of the lectins, to evaluate their role in triggering the obtained neutrophils phenotypes. Also, we will use models of infection in mice that were knocked out for TNF receptor, TLRs, dectin, and eventually others, with the purpose of identifying the primary neutrophils targets for the pathogens or their lectins. Finally, gene expression analysis of infected or lectin-stimulated neutrophils will allow to precisely determining the mechanisms involved in the interaction of neutrophils with the studied pathogens. By understanding the determinant mechanisms of the interactions established by innate immune receptors on neutrophils and pathogens components, it will be possible to create new therapeutic strategies against infectious diseases, in particular against the caused by T. gondii and P. brasiliensis. (AU) | |
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