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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

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Tomazi, Tiago [1] ; Goncalves, Juliano Leonel [1] ; Barreiro, Juliana Regina [1] ; de Campos Braga, Patricia Aparecida [2] ; Prada e Silva, Luis Felipe [1] ; Eberlin, Marcos Nogueira [2] ; dos Santos, Marcos Veiga [1]
Total Authors: 7
[1] Sch Vet Med & Anim Sci USP, Dept Anim Nutr & Prod, Sao Paulo - Brazil
[2] Univ Campinas UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Clinical Microbiology; v. 52, n. 5, p. 1658-1663, MAY 2014.
Web of Science Citations: 33

Coagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient method that can differentiate the bacterial species comprising this group. The aim of this study was to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to accurately identify CoNS species in dairy cow IMI. In addition, the study aimed to determine the frequency of CoNS species causing bovine IMI. A total of 108 bacterial isolates were diagnosed as CoNS by microbiological cultures from two milk samples collected from 21 dairy herds; the first sample was collected at the cow level (i.e., 1,242 composite samples from all quarters), while the second sample was collected at the mammary quarter level (i.e., 1,140 mammary samples collected from 285 cows). After CoNS isolation was confirmed by microbiological culture for both samples, all CoNS isolates (n= 108) were genotypically differentiated by PCR restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and subjected to the MALDI-TOF MS identification procedure. MALDI-TOF MS correctly identified 103 (95.4%) of the CoNS isolates identified by PCR-RFLP at the species level. Eleven CoNS species isolated from bovine IMI were identified by PCR-RFLP, and the most prevalent species was Staphylococcus chromogenes (n = 80; 74.1%). In conclusion, MALDI-TOF MS may be a reliable alternative method for differentiating CoNS species causing bovine IMI. (AU)

FAPESP's process: 11/04063-1 - Production and milk composition in dairy cows with mastitis caused by coagulase negative staphylococci
Grantee:Tiago Tomazi
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 11/14284-5 - Milk yield and composition in cows with mastitis caused by minor pathogens
Grantee:Marcos Veiga dos Santos
Support Opportunities: Regular Research Grants