Efficacy of citric acid denture cleanser on the Candida albicans biofilm formed on poly(methyl methacrylate): effects on residual biofilm and recolonization process

Author(s)

Faot, Fernanda ^[1] ; Cavalcanti, Yuri Wanderley ^[2] ; de Mendonca e Bertolini, Martinna ^[2] ; Pinto, Luciana de Rezende ^[1] ; da Silva, Wander Jose ^[2] ; Del Bel Cury, Altair Antoninha ^[2]

Affiliation

^[1] Univ Fed Pelotas, Sch Dent, Dept Prosthodont, BR-96015560 Rio Grande, Do Sul - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, BR-13414903 Sao Paulo - Brazil

• Document type: Journal article
• Source: BMC ORAL HEALTH; v. 14, JUN 23 2014.
• Web of Science Citations: 10

Abstract

Background: It is well known that the use of denture cleansers can reduce Candida albicans biofilm accumulation; however, the efficacy of citric acid denture cleansers is uncertain. In addition, the long-term efficacy of this denture cleanser is not well established, and their effect on residual biofilms is unknown. This in vitro study evaluated the efficacy of citric acid denture cleanser treatment on C. albicans biofilm recolonization on poly(methyl methacrylate) (PMMA) surface. Methods: C. albicans biofilms were developed for 72 h on PMMA resin specimens (n = 168), which were randomly assigned to 1 of 3 cleansing treatments (CTs) overnight (8 h). CTs included purified water as a control (CTC) and two experimental groups that used either a 1: 5 dilution of citric acid denture cleanser (CT5) or a 1: 8 dilution of citric acid denture cleanser (CT8). Residual biofilms adhering to the specimens were collected and quantified at two time points: immediately after CTs (ICT) and after cleaning and residual biofilm recolonization (RT). Residual biofilms were analyzed by quantifying the viable cells (CFU/mL), and biofilm architecture was evaluated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Denture cleanser treatments and evaluation periods were considered study factors. Data were analyzed using two-way ANOVA and Tukey's Honestly Significant Difference (HSD) test (alpha = 0.05). Results: Immediately after treatments, citric acid denture cleansing solutions (CT5 and CT8) reduced the number of viable cells as compared with the control (p < 0.01). However, after 48 h, both CT groups (CT5 and CT8) showed biofilm recolonization (p < 0.01). Residual biofilm recolonization was also detected by CLSM and SEM analysis, which revealed a higher biomass and average biofilm thickness for the CT8 group (p < 0.01). Conclusion: Citric acid denture cleansers can reduce C. albicans biofilm accumulation and cell viability. However, this CT did not prevent biofilm recolonization. (AU)

• FAPESP's process: 12/21011-8 - Growth conditions influence at structural and virulence characteristics of Candida and Streptococcus biofilms developed on in vitro models of human oral mucosa
• Grantee: Martinna de Mendonça e Bertolini
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/07436-6 - Analysis of the virulence of Candida albicans biofilms developed under different conditions
• Grantee: Yuri Wanderley Cavalcanti
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)