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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular detection of Leishmania spp. in road-killed wild mammals in the Central Western area of the State of Sao Paulo, Brazil

Full text
Author(s):
Richini-Pereira, Virginia Bodelao [1] ; Marson, Pamela Merlo [1] ; Hayasaka, Enio Yoshinori [1] ; Victoria, Cassiano [1] ; da Silva, Rodrigo Costa [1] ; Langoni, Helio [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, UNESP, Dept Higiene Vet & Saude Publ, Fac Med Vet & Zootecnia, Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 20, JUN 16 2014.
Web of Science Citations: 9
Abstract

Background: Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens as Leishmania spp., offering new opportunities for epidemiological studies of this infection. Methods: This study aimed to evaluate the presence of Leishmania spp. and Leishmania chagasi DNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals. Results: DNA was detected in tissues of one Cavia aperea (Brazilian guinea pig), five Cerdocyon thous (crab-eating fox), one Dasypus septemcinctus (seven-banded armadillo), two Didelphis albiventris (white-eared opossum), one Hydrochoerus hydrochoeris (capybara), two Myrmecophaga tridactyla (giant anteater), one Procyon cancrivorus (crab-eating raccoon), two Sphiggurus spinosus (porcupine) and one Tamandua tetradactyla (lesser anteater) from different locations in the Central Western part of Sao Paulo state. The Leishmania chagasi DNA were confirmed in mesenteric lymph node of one Cerdocyon thous. Results indicated common infection in wild animals. Conclusions: The approach employed herein proved useful for detecting the environmental occurrence of Leishmania spp. and L. chagasi, as well as determining natural wild reservoirs and contributing to understand the host-parasite interaction. (AU)

FAPESP's process: 08/09378-8 - MOLECULAR ECOEPIDEMIOLOGY OF MICROORGANISMS OF PUBLIC HEALTH INTEREST IN ROAD-KILLED WILD ANIMALS MID-WEST REGION OF THE STATE OF SÃO PAULO.
Grantee:Helio Langoni
Support Opportunities: Regular Research Grants
FAPESP's process: 08/08291-6 - MOLECULAR ECOEPIDEMIOLOGY OF MICROORGANISMS OF PUBLIC HEALTH INTEREST IN ROAD-KILLED WILD ANIMALS MID-WEST REGION OF THE STATE OF SÃO PAULO
Grantee:Virgínia Bodelão Richini Pereira
Support Opportunities: Scholarships in Brazil - Post-Doctoral