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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic analysis of p16 gene by PCR-SSCP technique and protein p16 expression in oral mucosa and skin melanomas

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Author(s):
Ricardo Hsieh [1] ; Fabrício Bitu Sousa ; Aline Firmiano [3] ; Fabio Daumas Nunes [4] ; Marina Helena Cury Gallottini de Magalhães [5] ; Mírian Nacagami Sotto [6]
Total Authors: 6
Affiliation:
[1] Universidade de São Paulo. Faculdade de Medicina - Brasil
[3] Universidade de São Paulo. Faculdade de Medicina. Departamento de Dermatologia - Brasil
[4] Universidade de São Paulo. Faculdade de Odontologia
[5] Universidade de São Paulo. Faculdade de Odontologia
[6] Universidade de São Paulo. Faculdade de Medicina. Departamento de Dermatologia - Brasil
Total Affiliations: 6
Document type: Journal article
Source: ANAIS BRASILEIROS DE DERMATOLOGIA; v. 81, n. 5, p. 433-441, 2006-10-00.
Abstract

BACKGROUND: Deletion and mutation of gene CDKN2a, which encodes a specific inhibitor of cyclin-dependent kinase 4 (CDK4), the protein p16, has been regarded as related to cutaneous melanoma tumorigenesis. However, little is known about those alterations in oral mucosa melanomas. OBJECTIVES: To verify possible p16 gene mutations and its protein expression in sporadic melanomas in oral mucosa and skin. MATERIALS AND METHODS: 36 primary sporadic melanoma paraffin-embedded specimens (seven oral mucosa and 29 skin lesions) were subjected to molecular analysis of exons 1, 2 and 3 of p16 gene using polymerase chain reaction/single strand conformational polymorphism technique. p16 protein expression was demonstrated by an immunohistochemical technique. Data obtained were correlated with tumor thickness. RESULTS: Five out of seven oral melanomas, and 17 out of 29 skin lesions displayed signs of alteration in p16 gene molecular analysis. Alterations in exon 2 of p16 gene were the most frequent. Protein p16 expression was observed in only one oral melanoma and in 10/13 (76.9%) skin melanomas up to 1.0 mm-thick and in 7/8 (87.5%) lesions thicker than 1.0 mm. CONCLUSIONS: Frequency of alterations disclosed by p16 gene molecular analysis in oral mucosa melanomas was 71.42% and 58.6% in cutaneous lesions. The obtained data suggest that p16 gene alterations play a role in the pathogenesis of sporadic melanoma of the oral mucosa. Neither protein p16 expression, nor p16 gene alteration had correlation with tumor thickness. (AU)