| Full text | |
| Author(s): |
Rodrigo Melo Gallindo
[1]
;
Frances Lilian Lanhellas Gonçalves
[2]
;
Rebeca Lopes Figueira
[3]
;
Ana Leda Bertoncini Simões
[4]
;
Lourenço Sbragia
[5]
Total Authors: 5
|
| Affiliation: | [1] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Surgery and Anatomy - Brasil
[2] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Surgery and Anatomy - Brasil
[3] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Surgery and Anatomy - Brasil
[4] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Surgery and Anatomy - Brasil
[5] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Surgery and Anatomy - Brasil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Rev. Col. Bras. Cir.; v. 41, n. 3, p. 181-187, 2014-06-00. |
| Abstract | |
OBJECTIVE: To standardize a technique for ventilating rat fetuses with Congenital Diaphragmatic Hernia (CDH) using a volume-controlled ventilator.METHODS: Pregnant rats were divided into the following groups: a) control (C); b) exposed to nitrofen with CDH (CDH); and c) exposed to nitrofen without CDH (N-). Fetuses of the three groups were randomly divided into the subgroups ventilated (V) and non-ventilated (N-V). Fetuses were collected on day 21.5 of gestation, weighed and ventilated for 30 minutes using a volume-controlled ventilator. Then the lungs were collected for histological study. We evaluated: body weight (BW), total lung weight (TLW), left lung weight (LLW), ratios TLW / BW and LLW / BW, morphological histology of the airways and causes of failures of ventilation.RESULTS: BW, TLW, LLW, TLW / BW and LLW / BW were higher in C compared with N- (p <0.05) and CDH (p <0.05), but no differences were found between the subgroups V and N-V (p> 0.05). The morphology of the pulmonary airways showed hypoplasia in groups N- and CDH, with no difference between V and N-V (p <0.05). The C and N- groups could be successfully ventilated using a tidal volume of 75 ìl, but the failure of ventilation in the CDH group decreased only when ventilated with 50 ìl.CONCLUSION: Volume ventilation is possible in rats with CDH for a short period and does not alter fetal or lung morphology. (AU) | |
| FAPESP's process: | 11/00794-1 - Assessment of nitric oxide (NO) in vivo after fetal tracheal occlusion on the expression of VEGF receptors in pulmonary congenital diaphragmatic hernia induced by Nitrofen |
| Grantee: | Lourenço Sbragia Neto |
| Support Opportunities: | Regular Research Grants |