Mechanical properties of mouse lungs along organ decellularization by sodium dodecyl sulfate

Author(s)

Nonaka, Paula N. ^{[1, 2, 3]} ; Uriarte, Juan J. ^{[4, 1]} ; Campillo, Noelia ^{[4, 5, 1]} ; Melo, Esther ^{[4, 1]} ; Navajas, Daniel ^{[4, 5, 1]} ; Farre, Ramon ^{[4, 1]} ; Oliveira, Luis V. F. ^{[2, 3]}

Affiliation

^[1] Univ Barcelona, Fac Med, Unitat Biofis & Bioengn, E-08007 Barcelona - Spain

^[2] Nove de Julho Univ, Masters Degree Program Rehabil Sci, Sao Paulo - Brazil

^[3] Nove de Julho Univ, Doctoral Degree Program Rehabil Sci, Sao Paulo - Brazil

^[4] CIBER Enfermedades Resp, Bunyola - Spain

^[5] Inst Bioengn Catalunya, Barcelona - Spain

• Document type: Journal article
• Source: Respiratory Physiology & Neurobiology; v. 200, p. 1-5, AUG 15 2014.
• Web of Science Citations: 16

Abstract

Lung decellularization is based on the use of physical, chemical, or enzymatic methods to break down the integrity of the cells followed by a treatment to extract the cellular material from the lung scaffold. The aim of this study was to characterize the mechanical changes throughout the different steps of lung decellularization process. Four lungs from mice (C57BL/6) were decellularized by using a conventional protocol based on sodium dodecyl sulfate. Lungs resistance (R-L) and elastance (E-L) were measured along decellularization steps and were computed by linear regression fitting of tracheal pressure, flow, and volume during mechanical ventilation. Transients differences found were more distinct in an intermediate step after the lungs were rinsed with deionized water and treated with 1% SDS, whereupon the percentage of variation reached approximately 80% for resistance values and 30% for elastance values. In conclusion, although a variation in extracellular matrix stiffness was observed during the decellularization process, this variation can be considered negligible overall because the resistance and elastance returned to basal values at the final decellularization step. (C) 2014 Published by Elsevier B.V. (AU)

• FAPESP's process: 12/04052-2 - Resistance and compliance of decellularized lung scaffolds along recellularization with stem cells
• Grantee: Paula Naomi Nonaka
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate