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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Endogenous hydrogen sulfide in the rostral ventrolateral medulla/Botzinger complex downregulates ventilatory responses to hypoxia

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Author(s):
Donatti, Alberto F. [1] ; Soriano, Renato N. [1] ; Sabino, Joao P. [1] ; Branco, Luiz G. S. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Physiol & Basic Pathol, BR-14040904 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Respiratory Physiology & Neurobiology; v. 200, p. 97-104, AUG 15 2014.
Web of Science Citations: 7
Abstract

Hydrogen sulfide (H2S) is now recognized as a new gaseous transmitter involved in several brain-mediated responses. The rostral ventrolateral medulla (RVLM)/Botzinger complex is a region in the brainstem that is involved in cardiovascular and respiratory functions. Recently, it has been shown that exogenous H2S in the RVLM modulates autonomic function and thus blood pressure. In the present study, we investigated whether H2S, endogenously produced in the RVLM/Botzinger complex, plays a role in the control of hypoxia-induced hyperventilation. Ventilation (V-E) was measured before and after bilateral microinjection of Na2S (H2S donor, 0.04, 1 and 2 pmol/100 nl) or aminooxyacetate (AOA, 0.2, 1 and 2 pmol/100 nl, a cystathionine beta-synthase, CBS, inhibitor) into the RVLM/Botzinger complex followed by a 60-min period of hypoxia (7% inspired O-2) or normoxia exposure. Control rats received microinjection of vehicle. Microinjection of vehicle, AOA or Na2S did not change V-E in normoxic conditions. Exposure to hypoxia evoked a typical increase in V-E. Microinjection of Na2S (2 pmol) followed by hypoxia exposure attenuated the hyperventilation. Conversely, microinjection of AOA (2 pmol) into the RVLM/Botzinger complex caused an increase in the hypoxia-induced hyperventilation. Thus, endogenous H2S in the RVLM/Botzinger complex seems to play no role in the maintenance of basal pulmonary ventilation during normoxia whereas during hypoxia H2S has a downmodulatory function. Homogenates of RVLM/Botzinger complex of animals previously exposed to hypoxia for 60 min exhibited a decreased rate of H2S production. Our data are consistent with the notion that the gaseous messenger H2S synthesis is downregulated in the RVLM/Botzinger complex during hypoxia favoring hyperventilation. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/01417-0 - Participation of hydrogen sulfide (H2S) on rostral ventrolateral medulla in the modulation of ventilatory responses to hypoxia
Grantee:Alberto Ferreira Donatti
Support Opportunities: Scholarships in Brazil - Post-Doctoral