Hepatitis A and E seroprevalence and associated risk factors: a community-based cross-sectional survey in rural Amazonia

Author(s)

Vitral, Claudia Lamarca ^{[1, 2]} ; da Silva-Nunes, Mnica ^[3] ; Pinto, Marcelo Alves ^[2] ; de Oliveira, Jaqueline Mendes ^[2] ; Coimbra Gaspar, Ana Maria ^[2] ; Costa Pereira, Rebeca Cristina ^[1] ; Ferreira, Marcelo Urbano ^[3]

Affiliation

^[1] Univ Fed Fluminense, Inst Biomed, Dept Microbiol & Parasitol, BR-24210130 Niteroi, RJ - Brazil

^[2] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Technol Dev Virol, Rio De Janeiro - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, BR-05508900 Sao Paulo - Brazil

• Document type: Journal article
• Source: BMC INFECTIOUS DISEASES; v. 14, AUG 23 2014.
• Web of Science Citations: 21

Abstract

Background: Hepatitis A virus (HAV) and hepatitis E virus (HEV) are both transmitted by the faecal-oral route, and represent common causes of acute hepatitis in developing countries. The endemicity of HAV infection has shifted from high to moderate in Brazil. Human cases of HEV infection seem to be rare, although the virus has been detected in swine livestock and effluents of slaughterhouses. This study was to determine the epidemiology of hepatitis A and E in one of the largest agricultural settlements in the Amazon Basin of Brazil. Methods: Serum samples collected from 397 individuals aged between 5 and 90 years during a population-based cross-sectional survey were tested for anti-HAV and anti-HEV antibodies. Associated risk factors and spatial clustering of HAV and HEV seropositivity were also analyzed. Results: The overall rate of HAV seropositivity was 82.9% (95% confidence interval (CI), 79.2-86.6%). Multilevel logistic regression analysis identified increasing age (in years; odds ratio (OR), 1.097; 95% CI, 1.050-1.147; P < 0.001) and crowding (OR, 1.603; 95% CI, 1.054-2.440; P = 0.028) as significant risk factors for HAV seropositivity. Anti-HEV IgG was detected in 50/388 settlers (12.9%, 95% CI, 9.5-16.2%). Anti-HEV IgM was detected in 7/43 (16.3%) anti-IgG positive samples, and 4 of them had a confirmed result by immunoblot. Increasing age was the only significant determinant of HEV seropositivity (OR, 1.033; 95% CI, 1.016-1.050; P < 0.001). No significant spatial clustering of HAV and HEV seropositivity was detected in the area. Conclusions: Both HAV and HEV are endemic, with differing rates of infection in children and adults in this rural setting of the Brazilian Amazon. Anti-HEV prevalence was considerably higher than those previously reported in Brazil. The detection of HEV- specific IgM antibodies in four asymptomatic individuals is highly suggestive of the circulation of HEV in this rural population. (AU)