| Texto completo | |
| Autor(es): Mostrar menos - |
Machado-Neto, Joao Agostinho
[1]
;
Lazarini, Mariana
[1]
;
Favaro, Patricia
[1]
;
Campos, Paula de Melo
[1]
;
Scopim-Ribeiro, Renata
[1]
;
Franchi Junior, Gilberto Carlos
[2]
;
Nowill, Alexandre Eduardo
[2]
;
Moura Lima, Paulo Roberto
[1]
;
Costa, Fernando Ferreira
[1]
;
Benichou, Serge
[3]
;
Olalla Saad, Sara Teresinha
[1]
;
Traina, Fabiola
[1]
Número total de Autores: 12
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Hematol & Hemotherapy Ctr, Hemoctr Unicamp, Inst Nacl Ciencia & Tecnol Sangue, BR-13083878 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Integrated Ctr Childhood Oncohematol Invest, BR-13083878 Campinas, SP - Brazil
[3] Inst Cochin, INSERM, U1016, Paris - France
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH; v. 1853, n. 3, p. 583-593, MAR 2015. |
| Citações Web of Science: | 10 |
| Resumo | |
ANKHD1 is highly expressed inhuman acute leukemia cells and potentially regulates multiple cellular functions through its ankyrin-repeat domains. In order to identify interaction partners of the ANKHD1 protein and its role in leukemia cells, we performed a yeast two-hybrid system screen and identified SIVA, a cellular protein known to be involved in proapoptotic signaling pathways. The interaction between ANKHD1 and SNA was confirmed by co-imunoprecipitation assays. Using human leukemia cell models and lentivirus-mediated shRNA approaches, we showed that ANKHD1 and SIVA proteins have opposing effects. While it is known that SIVA silencing promotes Stathmin 1 activation, increased cell migration and xenograft tumor growth, we showed that ANKHD1 silencing leads to Stathmin 1 inactivation, reduced cell migration and xenograft tumor growth, likely through the inhibition of SIVA/Stathmin 1 association. In addition, we observed that ANKHD1 knockdown decreases cell proliferation, without modulating apoptosis of leukemia cells, while SIVA has a proapoptotic function in U937 cells, but does not modulate proliferation in vitro. Results indicate that ANKHD1 binds to SIVA and has an important role in inducing leukemia cell proliferation and migration via the Stathmin 1 pathway. ANKHD1 may be an oncogene and participate in the leukemia cell phenotype. (C) 2014 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 09/00268-8 - Estudo da função de ANKHD1 na apoptose e sua interação com SIVA em células neoplásicas |
| Beneficiário: | Fabíola Traina |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/51959-0 - Biologia das doenças neoplásicas da medula óssea |
| Beneficiário: | Sara Teresinha Olalla Saad |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 11/06840-5 - Estudo da função de ANKHD1 na proliferação, apoptose e ciclo celular em neoplasias hematológicas |
| Beneficiário: | João Agostinho Machado Neto |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 12/09982-8 - Investigação de alterações moleculares em neoplasias mieloides. |
| Beneficiário: | Fabíola Traina |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |