NS1 codon usage adaptation to humans in pandemic Zika virus

Autor(es):Mostrar menos -

Caio César de Melo Freire ^[1] ; Giuseppe Palmisano ^[2] ; Carla T Braconi ^[3] ; Fernanda R Cugola ^[4] ; Fabiele B Russo ^[4] ; Patricia CB Beltrão-Braga ; Atila Iamarino ^[7] ; Daniel Ferreira de Lima Neto ^[3] ; Amadou Alpha Sall ^[7] ; Livia Rosa-Fernandes ^[8] ; Martin R Larsen ^[8] ; Paolo Marinho de Andrade Zanotto ^[3]

Afiliação do(s) autor(es)

^[1] Universidade Federal de São Carlos. Departamento de Genética e Evolução - Brasil

^[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Lab Evolucao Mol & Bioinforrnat, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Lab Celulas Tronco, Dept Cirurgia, Sao Paulo, SP - Brazil

^[6] Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia - Brasil

^[7] Inst Pasteur, Dakar - Senegal

^[8] Univ Southern Denmark, Dept Biochem & Mol Biol, Odense - Denmark

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 113, n. 5 2018-05-10.

Resumo

BACKGROUND Zika virus (ZIKV) was recognised as a zoonotic pathogen in Africa and southeastern Asia. Human infections were infrequently reported until 2007, when the first known epidemic occurred in Micronesia. After 2013, the Asian lineage of ZIKV spread along the Pacific Islands and Americas, causing severe outbreaks with millions of human infections. The recent human infections of ZIKV were also associated with severe complications, such as an increase in cases of Guillain-Barre syndrome and the emergence of congenital Zika syndrome. OBJECTIVES To better understand the recent and rapid expansion of ZIKV, as well as the presentation of novel complications, we compared the genetic differences between the African sylvatic lineage and the Asian epidemic lineage that caused the recent massive outbreaks. FINDINGS The epidemic lineages have significant codon adaptation in NS1 gene to translate these proteins in human and Aedes aegypti mosquito cells compared to the African zoonotic lineage. Accordingly, a Brazilian epidemic isolate (ZBR) produced more NS1 protein than the MR766 African lineage (ZAF) did, as indicated by proteomic data from infections of neuron progenitor cells-derived neurospheres. Although ZBR replicated more efficiently in these cells, the differences observed in the stoichiometry of ZIKV proteins were not exclusively explained by the differences in viral replication between the lineages. MAIN CONCLUSIONS Our findings suggest that natural, silent translational selection in the second half of 20th century could have improved the fitness of Asian ZIKV lineage in human and mosquito cells. (AU)

• Processo FAPESP: 14/06090-4 - Estimativa da diversidade intra e inter populacional gênica e genética do baculovírus Anticarsia gemmatalis múltiplo nucleopoliedrovírus (AgMNPV) em isolados de campo na América do Sul
• Beneficiário: Caio César de Melo Freire
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/03605-9 - Expressão temporal dos genes do Zika vírus das linhagens africana e brasileira e mapeamento da expressão gênica em sistemas permissivos, semi-permissivos e não permissivos
• Beneficiário: Daniel Ferreira de Lima Neto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/17766-9 - Abordagem sistêmica no estudo da permissividade do Anticarsia gemmatalis múltiplo nucleopoliedrovírus (AgMNPV)
• Beneficiário: Paolo Marinho de Andrade Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/04818-5 - Evolução e filodinâmica dos recombinantes BF de HIV-1 da América do Sul
• Beneficiário: Atila Iamarino
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/03911-7 - Avaliação da interação parasita-hospedeiro do baculovírus AgMNPV em linhagens celulares distintas de Lepidópteras
• Beneficiário: Carla Torres Braconi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado