Migration and proliferation of cancer cells in culture are differentially affected by molecular size of modified citrus pectin

Autor(es)

Ramos do Prado, Samira Bernardino ^[1] ; Shiga, Tania Misuzu ^[1] ; Harazono, Yosuke ^{[2, 3, 4, 5]} ; Hogan, Victor A. ^{[2, 3, 4]} ; Raz, Avraham ^{[2, 3, 4]} ; Carpita, Nicholas C. ^[6] ; Fabi, Joao Paulo ^{[7, 1, 8]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food Sci & Expt Nutr, Sao Paulo, SP - Brazil

^[2] Wayne State Univ, Sch Med, Dept Oncol, Detroit, MI - USA

^[3] Wayne State Univ, Sch Med, Dept Pathol, Detroit, MI 48201 - USA

^[4] Karmanos Canc Inst, B, Detroit, MI - USA

^[5] Tokyo Med & Dent Univ, Dept Maxillofacial Surg, Bunkyo Ku, Tokyo - Japan

^[6] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 - USA

^[7] Univ Sao Paulo, Food & Nutr Res Ctr NAPAN, Sao Paulo, SP - Brazil

^[8] Sao Paulo Res Fdn, Res Innovat & Disseminat Cts, CEPID FAPESP, Food Res Ctr FoRC, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Carbohydrate Polymers; v. 211, p. 141-151, MAY 1 2019.
• Citações Web of Science: 4

Resumo

While chemically and thermally modified citrus pectin (MCP) has already been studied for health benefits, it is unknown how size-fractionated oligo-and polysaccharides differentially affect cancer cell behavior. We produced thermally MCP and fractionated it by molecular size to evaluate the effect these polymers have on cancer cells. MCP30/10 (between 30 and 10 kDa) had more esterified homogalacturonans (HG) and fewer rhamnogalacturonans (RG-I) than MCP and MCP30 (higher than 30 kDa), while MCP10/3 (between 10 and 3 kDa) showed higher amounts of type I arabinogalactans (AGI) and lower amounts of RG-I. MCP3 (smaller than 3 kDa) presented less esterified HG and the lowest amount of AGI and RG-I. Our data indicate that the enrichment of deesterified HG oligomers and the AGI and RG-I depletions in MCP3, or the increase of AGI and loss of RGI in MCP30/10, enhance the anticancer behaviors by inhibiting migration, aggregation, and proliferation of cancer cells. (AU)

• Processo FAPESP: 15/01004-5 - Equipamento multiusuário concedido no processo 2012/23970-2: QuantStudio 7 flex Real-Time PCR system, 96-well fast, laptop (nº catálogo 4485698)
• Beneficiário: Joao Paulo Fabi
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/23970-2 - Alterações biológicas das pectinas de mamão com possíveis benefícios à saúde humana
• Beneficiário: Joao Paulo Fabi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores