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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Migration and proliferation of cancer cells in culture are differentially affected by molecular size of modified citrus pectin

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Ramos do Prado, Samira Bernardino [1] ; Shiga, Tania Misuzu [1] ; Harazono, Yosuke [2, 3, 4, 5] ; Hogan, Victor A. [2, 3, 4] ; Raz, Avraham [2, 3, 4] ; Carpita, Nicholas C. [6] ; Fabi, Joao Paulo [7, 1, 8]
Total Authors: 7
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food Sci & Expt Nutr, Sao Paulo, SP - Brazil
[2] Wayne State Univ, Sch Med, Dept Oncol, Detroit, MI - USA
[3] Wayne State Univ, Sch Med, Dept Pathol, Detroit, MI 48201 - USA
[4] Karmanos Canc Inst, B, Detroit, MI - USA
[5] Tokyo Med & Dent Univ, Dept Maxillofacial Surg, Bunkyo Ku, Tokyo - Japan
[6] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 - USA
[7] Univ Sao Paulo, Food & Nutr Res Ctr NAPAN, Sao Paulo, SP - Brazil
[8] Sao Paulo Res Fdn, Res Innovat & Disseminat Cts, CEPID FAPESP, Food Res Ctr FoRC, Sao Paulo, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Source: Carbohydrate Polymers; v. 211, p. 141-151, MAY 1 2019.
Web of Science Citations: 4

While chemically and thermally modified citrus pectin (MCP) has already been studied for health benefits, it is unknown how size-fractionated oligo-and polysaccharides differentially affect cancer cell behavior. We produced thermally MCP and fractionated it by molecular size to evaluate the effect these polymers have on cancer cells. MCP30/10 (between 30 and 10 kDa) had more esterified homogalacturonans (HG) and fewer rhamnogalacturonans (RG-I) than MCP and MCP30 (higher than 30 kDa), while MCP10/3 (between 10 and 3 kDa) showed higher amounts of type I arabinogalactans (AGI) and lower amounts of RG-I. MCP3 (smaller than 3 kDa) presented less esterified HG and the lowest amount of AGI and RG-I. Our data indicate that the enrichment of deesterified HG oligomers and the AGI and RG-I depletions in MCP3, or the increase of AGI and loss of RGI in MCP30/10, enhance the anticancer behaviors by inhibiting migration, aggregation, and proliferation of cancer cells. (AU)

FAPESP's process: 15/01004-5 - Multi-User Equipment approved in grant 2012/23970-2: QuantStudio 7 flex Real-Time PCR system, 96-well fast, laptop (catalog #: 4485698)
Grantee:Joao Paulo Fabi
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 13/07914-8 - FoRC - Food Research Center
Grantee:Bernadette Dora Gombossy de Melo Franco
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/23970-2 - Biological changes of papaya pectins with possible benefits to human health
Grantee:Joao Paulo Fabi
Support Opportunities: Research Grants - Young Investigators Grants