Impacts of in vitro gastrointestinal digestion on phenolic compounds bioaccessibility and biological activities of uvaia (Eugenia pyriformis Cambess) fractions

Autor(es)

Farias, David de Paulo ; de Araujo, Fabio Fernandes ; Sawaya, Alexandra C. H. F. ; Neri-Numa, Iramaia Angelica ; Pastore, Glaucia Maria

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 153, p. 11-pg., 2025-06-01.

Resumo

Uvaia (Eugenia pyriformis Cambess) is a species native to the Atlantic Rainforest (Brazil) that has a great potential for technological applications, but which remains unexplored in relation to its biological potential. Hereby, we evaluated the effect of in vitro gastrointestinal digestion on phenolic compounds bioaccessibility, antioxidant capacity, alpha-amylase, alpha-glucosidase inhibition and antiglycation activity of two uvaia portions: edible fraction and seed. The phenolic characterization, antioxidant capacity and antidiabetic potential of the aqueous phenolic extract of both uvaia fractions were also evaluated. UHPLC-MS/MS analysis in the aqueous extract showed that quinic acid was the main organic acid present in uvaia seed (171.91 mg/100 g), whereas quercitrin was found mainly in the edible fraction (16.00 mg/100 g). Uvaia seed had the greatest antioxidant capacity (9951 - 37500 mu mol Trolox equivalent (TE)/100 g) compared with edible fraction (1900 - 9123 mu mol TE/100 g). Edible fraction showed higher alpha-amylase inhibitory potential (IC50 = 5.72 mg/mL), while there was no difference between both evaluated fractions for alpha- glucosidase inhibition (IC50 = 5.24-5.26 mg/mL). Uvaia seed showed higher antiglycation potential (IC50 = 1.09 mg/mL) compared with the edible fraction (IC50 = 8.46 mg/mL). Regarding the phenolics bioaccessibility, rutin and epicatechin significantly decreased after gastrointestinal digestion for both fractions. However, p-coumaric acid and quercitrin significantly increased after digestion for edible fraction (7 and 3-fold, respectively). In general, the antidiabetic potential of both uvaia fractions increased after gastrointestinal digestion. These results could encourage the development of new formulations with functional claims using different parts of uvaia. (AU)

• Processo FAPESP: 20/00225-6 - Determinação do potencial antioxidante, bioacessibilidade in vitro e avaliação das atividades antidiabética e prebiótica da uvaia (Eugenia pyriformis Cambess)
• Beneficiário: David de Paulo Farias
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 20/15163-6 - Determinação da composição química, bioacessibilidade e avaliação das atividades antidiabéticas e prebióticas dos frutos de Xique-Xique (Pilosocereus gounellei) e Mandacaru (Cereus jamacaru)
• Beneficiário: Fábio Fernandes de Araújo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 22/05242-1 - Efeito da fermentação colônia sobre o potencial antidiabético da uvaia (Eugenia pyriformis Cambess.)
• Beneficiário: David de Paulo Farias
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/50333-1 - Cobra: nova plataforma de descoberta de compostos para identificação rápida e de baixo custo de bioativo com efeitos benéficos originários de plantas brasileiras
• Beneficiário: Glaucia Maria Pastore
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 22/04530-3 - Potencial antidiabético dos frutos de xique-xique (Pilosocereus gounellei) e mandacaru (Cereus jamacaru) após fermentação colônica
• Beneficiário: Fábio Fernandes de Araújo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado