Fast analysis of beta-ecdysone in Brazilian ginseng (Pfaffia glomerata) extracts by high-performance liquid chromatography using a fused-core column

Autor(es)

Rostagno, Mauricio A. ^[1] ; Debien, Isabel C. N. ^[1] ; Vardanega, Renata ^[1] ; Nogueira, Gislaine C. ^[1] ; Barbero, Gerardo F. ^[2] ; Meireles, M. Angela A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Sch Food Engn, LASEFI, DEA, FEA, BR-13083862 Campinas, SP - Brazil

^[2] Univ Cadiz, Agrifood Campus Int Excellence CeiA3, Fac Sci, Analyt Chem Dept, Cadiz 11510 - Spain

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL METHODS; v. 6, n. 8, p. 2452-2459, 2014.
• Citações Web of Science: 10

Resumo

The recent development of fused-core technology in HPLC columns is enabling faster and highly efficient separations. This technology was evaluated for the development of a fast analysis method for beta-ecdysone in extracts of Pfaffia glomerata. A step-by-step strategy was used to optimize temperature (30-55 degrees C), flow rate (1.0-2.0 mL min(-1)), mobile phase composition (mixtures of water and methanol or acetonitrile) and equilibration time (1-5 min). A gradient method has been developed using two solvents: 0.1% acetic acid in water and 0.1% acetic acid in acetonitrile. Optimized conditions provided a method for the separation of beta-ecdysone in approximately 2 min with a total analysis time (sample-to-sample) of 9 min, including the return to initial conditions and the re-equilibration of the column. Evaluation of chromatographic performance revealed excellent intraday and interday reproducibility (>99.5%), resolution (2.78), selectivity (1.13), and peak symmetry (1.09) while presenting low limits of detection (0.20 mg L-1) and quantitation (0.67 mg L-1). The robustness of the method has also been calculated according to the concentration/dilution of the sample. Several sample solvents were evaluated and the best chromatographic results were obtained using 80% methanol in water. Finally, the developed method was validated with different extracts of Pfaffia glomerata samples. (AU)

• Processo FAPESP: 13/04304-4 - Desenvolvimento de sistemas integrados para a análise de compostos bioativos em produtos naturais utilizando tecnologia supercrítica
• Beneficiário: Maurício Ariel Rostagno
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/10685-8 - Combinação/acoplamento de processos para a produção e encapsulamento de extratos vegetais de alto valor agregado: avaliação técnica e econômica
• Beneficiário: Maria Angela de Almeida Meireles Petenate
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/15049-5 - Desenvolvimento de sistemas integrados para a análise de compostos bioativos em produtos naturais utilizando tecnologia supercrítica
• Beneficiário: Maurício Ariel Rostagno
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores