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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fast analysis of beta-ecdysone in Brazilian ginseng (Pfaffia glomerata) extracts by high-performance liquid chromatography using a fused-core column

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Author(s):
Rostagno, Mauricio A. [1] ; Debien, Isabel C. N. [1] ; Vardanega, Renata [1] ; Nogueira, Gislaine C. [1] ; Barbero, Gerardo F. [2] ; Meireles, M. Angela A. [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Sch Food Engn, LASEFI, DEA, FEA, BR-13083862 Campinas, SP - Brazil
[2] Univ Cadiz, Agrifood Campus Int Excellence CeiA3, Fac Sci, Analyt Chem Dept, Cadiz 11510 - Spain
Total Affiliations: 2
Document type: Journal article
Source: ANALYTICAL METHODS; v. 6, n. 8, p. 2452-2459, 2014.
Web of Science Citations: 10
Abstract

The recent development of fused-core technology in HPLC columns is enabling faster and highly efficient separations. This technology was evaluated for the development of a fast analysis method for beta-ecdysone in extracts of Pfaffia glomerata. A step-by-step strategy was used to optimize temperature (30-55 degrees C), flow rate (1.0-2.0 mL min(-1)), mobile phase composition (mixtures of water and methanol or acetonitrile) and equilibration time (1-5 min). A gradient method has been developed using two solvents: 0.1% acetic acid in water and 0.1% acetic acid in acetonitrile. Optimized conditions provided a method for the separation of beta-ecdysone in approximately 2 min with a total analysis time (sample-to-sample) of 9 min, including the return to initial conditions and the re-equilibration of the column. Evaluation of chromatographic performance revealed excellent intraday and interday reproducibility (>99.5%), resolution (2.78), selectivity (1.13), and peak symmetry (1.09) while presenting low limits of detection (0.20 mg L-1) and quantitation (0.67 mg L-1). The robustness of the method has also been calculated according to the concentration/dilution of the sample. Several sample solvents were evaluated and the best chromatographic results were obtained using 80% methanol in water. Finally, the developed method was validated with different extracts of Pfaffia glomerata samples. (AU)

FAPESP's process: 13/15049-5 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology
Grantee:Maurício Ariel Rostagno
Support type: Scholarships in Brazil - Young Researchers
FAPESP's process: 13/04304-4 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology
Grantee:Maurício Ariel Rostagno
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 12/10685-8 - Combination and coupling of processes for the production and encapsulation of value-added vegetal extracts: technical and economic evaluation
Grantee:Maria Angela de Almeida Meireles Petenate
Support type: Regular Research Grants