Multi user equipment approved in grant 2018/14582-5: ultra-high pressure liquid ch...
| Full text | |
| Author(s): |
Rostagno, Mauricio A.
[1]
;
Debien, Isabel C. N.
[1]
;
Vardanega, Renata
[1]
;
Nogueira, Gislaine C.
[1]
;
Barbero, Gerardo F.
[2]
;
Meireles, M. Angela A.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Campinas, UNICAMP, Sch Food Engn, LASEFI, DEA, FEA, BR-13083862 Campinas, SP - Brazil
[2] Univ Cadiz, Agrifood Campus Int Excellence CeiA3, Fac Sci, Analyt Chem Dept, Cadiz 11510 - Spain
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | ANALYTICAL METHODS; v. 6, n. 8, p. 2452-2459, 2014. |
| Web of Science Citations: | 10 |
| Abstract | |
The recent development of fused-core technology in HPLC columns is enabling faster and highly efficient separations. This technology was evaluated for the development of a fast analysis method for beta-ecdysone in extracts of Pfaffia glomerata. A step-by-step strategy was used to optimize temperature (30-55 degrees C), flow rate (1.0-2.0 mL min(-1)), mobile phase composition (mixtures of water and methanol or acetonitrile) and equilibration time (1-5 min). A gradient method has been developed using two solvents: 0.1% acetic acid in water and 0.1% acetic acid in acetonitrile. Optimized conditions provided a method for the separation of beta-ecdysone in approximately 2 min with a total analysis time (sample-to-sample) of 9 min, including the return to initial conditions and the re-equilibration of the column. Evaluation of chromatographic performance revealed excellent intraday and interday reproducibility (>99.5%), resolution (2.78), selectivity (1.13), and peak symmetry (1.09) while presenting low limits of detection (0.20 mg L-1) and quantitation (0.67 mg L-1). The robustness of the method has also been calculated according to the concentration/dilution of the sample. Several sample solvents were evaluated and the best chromatographic results were obtained using 80% methanol in water. Finally, the developed method was validated with different extracts of Pfaffia glomerata samples. (AU) | |
| FAPESP's process: | 13/15049-5 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology |
| Grantee: | Maurício Ariel Rostagno |
| Support type: | Scholarships in Brazil - Young Researchers |
| FAPESP's process: | 13/04304-4 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology |
| Grantee: | Maurício Ariel Rostagno |
| Support type: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 12/10685-8 - Combination and coupling of processes for the production and encapsulation of value-added vegetal extracts: technical and economic evaluation |
| Grantee: | Maria Angela de Almeida Meireles Petenate |
| Support type: | Regular Research Grants |