Genetic and molecular basis of chlorophyll in seeds: a step forward to improve soybean adaptability to climate change

• Grant number: 17/50211-9
• Support Opportunities: Research Projects - Thematic Grants
• Duration: May 01, 2018 - April 30, 2023
• Field of knowledge: Biological Sciences - Biology
• Convênio/Acordo: Netherlands Organisation for Scientific Research (NWO)
• Principal Investigator: Edvaldo Aparecido Amaral da Silva
• Grantee: Edvaldo Aparecido Amaral da Silva
• Principal researcher abroad: Henk Wilhelmus Maria Hilhorst
• Institution abroad: Wageningen University, Netherlands
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 22/11417-9 - Identification of SNP (Single Nucleotide Polymorphisms) markers in genomic regions associated with the regulation of chlorophyll degradation in soybean seeds, BP.PD; 21/03209-4 - How are the transcription factors ABI3 and ABI5 associated with chlorophyll retention in soybean seeds?, BP.IC; 20/12405-9 - Genetic and molecular basis of chlorophyll retention in seeds: a step forward to improve soybean adaptability to climate change, BP.PD; 19/06160-6 - Influence of the environment on the occurrence of green seeds, production of oil and protein in a soybean genotype with accumulation of ß-Carotene, BP.DR

Abstract

Due to global climate change, agricultural areas in the world are increasingly prone to climatic extremes. For most species, extreme temperatures and drought stress affects seed quality. This is particularly problematic in oilseeds, such as soybean and canola, and often results in what is known as the "green seed problem". This is when chlorophyll is retained in ripening seeds, resulting in lower oil and seed quality. Despite its considerable importance for seed quality and food security, the mechanisms underlying chlorophyll degradation in oilseeds are poorly understood, which is a major limiting facto r in research into applied solutions to the green seed problem. Over the past years, our groups have accumulated fundamental and applied knowledge on chlorophyll retention in seeds and a solid and fruitful collaboration between Wageningen University and Research and Brazilian research institutes Universidade Estadual Paulista (UNESP) and Embrapa-Soja, both responsible for the transfer of fundamental knowledge into crops. Now we aim to use this knowledge to identify the main regulators of chlorophyll degradation in seeds, to understand the biological significance of chlorophyll in seeds and identify loci responsible for chlorophyll degradation in soybean seeds. For this, we propose to use a combination of physiological, genetic and molecular approaches using a model plant to shed Iight on the molecular regulatory networks of chlorophyll degradation in seeds, concomitantly with a GBS-GWAS approach that will be used in soybean to allow the association between fundamental and applied research. The identification of genetic and molecular markers will greatly aid plant breeders in selecting varieties with desired oil, protein and seed quality traits, together with a low susceptibility to green seed formation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BATISTA, THIAGO BARBOSA; PERISSATO, SAMARA MOREIRA; QUEIROZ REGO, CARLOS HENRIQUE; FONSECA DE OLIVEIRA, GUSTAVO ROBERTO; HENNING, FERNANDO AUGUSTO; AMARAL DA SILVA, EDVALDO APARECIDO. Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?. JOURNAL OF SEED SCIENCE, v. 43, 2021. (17/50211-9, 19/06160-6)

RIBEIRO-OLIVEIRA, JOAO PAULO; SILVEIRA, LILIAN E. D.; PINTO, LILIAN V. A.; SILVA, EDVALDO A. A.; HILHORST, HENK W. M.. Clues on an intraspecific communication system in seed-seedling transition. PHYSIOLOGIA PLANTARUM, v. 172, n. 3, p. 1609-1618, JUL 2021. (17/50211-9, 20/12405-9)

ZINSMEISTER, JULIA; BERRIRI, SOUHA; BASSO, DENISE PUNTEL; LY-VU, BENOIT; DANG, THI-THU; LALANNE, DAVID; AMARAL DA SILVA, EDVALDO APARECIDO; LEPRINCE, OLIVIER; BUITINK, JULIA. The seed-specific heat shock factorA9regulates the depth of dormancy inMedicago truncatulaseeds viaABAsignalling. PLANT CELL AND ENVIRONMENT, v. 43, n. 10, AUG 2020. (17/50211-9)