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Influence of the environment on the occurrence of green seeds, production of oil and protein in a soybean genotype with accumulation of ß-Carotene

Grant number: 19/06160-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2019
Effective date (End): July 31, 2022
Field of knowledge:Agronomical Sciences - Agronomy - Crop Science
Principal Investigator:Edvaldo Aparecido Amaral da Silva
Grantee:Thiago Barbosa Batista
Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated research grant:17/50211-9 - Genetic and molecular basis of chlorophyll in seeds: a step forward to improve soybean adaptability to climate change, AP.TEM

Abstract

The aim is to evaluate the impact of environmental conditions on green seed tolerance, yield and quality of oil and protein in a soybean genotype with increased ß-carotene synthesis. The plants will be grown in greenhouse in the absence of stress with free water supplement (field capacity) and average temperature of 24.7ºC. At stage R5.1, the plants will be maintained under these conditions and alternately transferred to stress conditions where the average temperature will vary from 25.7 to 39.5°C and the soil water potential will be -0.1 MPa. The flowers will be labeled and the seeds in development will be harvested manually from the R6 to the R9 stage. The collected seeds will be analyzed for germination, vigor, desiccation tolerance, longevity, retention of chlorophyll, abscisic acid content, oil quantity and quality, tocopherol and chlorophyll content in oil and protein. In addition, gene expression will be studied with genes already described as involved in the degradation of chlorophyll. To indicate environmental stress, the content of malondialdehyde and hydrogen peroxide will be evaluated at the R6 stage. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BATISTA, THIAGO BARBOSA; PERISSATO, SAMARA MOREIRA; QUEIROZ REGO, CARLOS HENRIQUE; FONSECA DE OLIVEIRA, GUSTAVO ROBERTO; HENNING, FERNANDO AUGUSTO; AMARAL DA SILVA, EDVALDO APARECIDO. Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?. JOURNAL OF SEED SCIENCE, v. 43, . (17/50211-9, 19/06160-6)
DUCATTI, KARINA RENOSTRO; BATISTA, THIAGO BARBOSA; HIRAI, WELINTON YOSHIO; LUCCAS, DAIANI AJALA; MORENO, LETICIA DE AGUILA; GUIMARAES, CRISTIANE CARVALHO; BASSEL, GEORGE W.; AMARAL DA SILVA, EDVALDO APARECIDO. Transcripts Expressed during Germination Sensu Stricto Are Associated with Vigor in Soybean Seeds. PLANTS-BASEL, v. 11, n. 10, p. 15-pg., . (17/50211-9, 19/06160-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BATISTA, Thiago Barbosa. Maturation in soybean seeds: reproductive stages, chlorophyll degradation and acquisition of physiological quality. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agronômicas. Botucatu Botucatu.

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