Full text
| |
| Author(s): |
Thiago Barbosa Batista
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Botucatu. 2022-12-20. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agronômicas. Botucatu |
| Defense date: | 2022-10-20 |
| Advisor: | Edvaldo Aparecido Amaral da Silva |
| Abstract | |
Seeds with superior physiological quality are those with the highest level of maturity. In soybean seeds, the attributes of physiological quality are acquired sequentially throughout maturation. In this legume, late maturation is an important phase in building the physiological potential of seeds. In this phase, an intense degradation of chlorophyll occurs. Despite the importance of knowing the maturation process, the identification of seeds at different stages of maturation to characterize the maturity of lots is generally subjective, as it is based on visual inspection. Furthermore, little is known about the relationship between chlorophyll degradation and the acquisition of physiological quality. Therefore, here we investigated (i) the possibility of segmenting soybean seeds at different maturation stages using spectral autofluorescence images and (ii) how chlorophyll degradation during soybean maturation impacts the acquisition of seed physiological quality. For this, we used spectral autofluorescence images integrated with artificial intelligence and a physiological and molecular approach during the maturation of soybean seeds. Our results demonstrate that (i) the use of spectral autofluorescence images at the wavelength of chlorophylls allows segmenting soybean seeds at each stage of maturation (i.e., R7.1, R7.2, R7.3, R8 and R9 ) bringing reliability to analyze the maturity level of seeds using these wavelengths, and (ii) chlorophyll degradation does not affect the acquisition of germination and seed vigor and, however, the longevity of soybean seeds cannot be decoupled from this process. (AU) | |
| FAPESP's process: | 19/06160-6 - Influence of the environment on the occurrence of green seeds, production of oil and protein in a soybean genotype with accumulation of ß-Carotene |
| Grantee: | Thiago Barbosa Batista |
| Support Opportunities: | Scholarships in Brazil - Doctorate |