Influence of the administratin of Luffa operculata on the corticosterone,TNF-±,IL-1,IL-6 and IL-10, testosterone,TGO,TGP and creatinin as indicatorsof behavioral alterations, over reproductive system, liver and kidneys of F0 and F1 Wistar rats.

• Grant number: 17/03470-9
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2018
• End date: March 31, 2019
• Field of knowledge: Biological Sciences - Botany - Applied Botany
• Principal Investigator: Ivana Barbosa Suffredini
• Grantee: Ivana Barbosa Suffredini
• Host Institution: Vice-Reitoria de Pesquisa e Pós-Graduação. Universidade Paulista (UNIP). São Paulo , SP, Brazil

Abstract

Buchinha-do-norte, or Luffa operculata, is a plant of popular use against sinusitis, used unorthodoxly by its abortive effects. The side effects of this species in pregnant women and in men who use it against sinusitis are still insipiently known and require further studies. The deleterious and teratogenic effects of this species have been reported, however, its residual effects in case of an unsuccessful abortion are not reported in the literature, neither are the possible influences on the male reproductive system. Thus, the present study aims at verifying changes in behavior after oral administration of a non-abortive dose of aqueous extract of buchinha-do-norte to male and female adult rats (F0) and their offspring (F1). Serum corticosterone and biochemical indicators, as well as histopathological evaluation of the brain, liver, kidneys and testes are going to be made. The buchinha-do-norte fruits were used to prepare the aqueous extract in the ratio of one buchinha to each 300 mL of boiling water. The aqueous extract will be cooled, filtered, frozen in a freezer (-70 ° C) and freeze-dried. F0 and F1 animals, both male and female, will be divided into two groups, control and test, considering the analysis within each category gender / age. The test groups will receive treatment with 3 mg / kg per day of aqueous extract of buchinha-do-norte, orally (gavage), while the animals in the control groups will receive water, also by gavage. Pregnant adult females will receive treatments between days 15 and 21 of gestation (GD15 and GD21). Adult males in the test group will be orally treated with 3 mg/kg per day of aqueous buchinha-do-norte, for five consecutive days, while those in the control group will receive water. Studies will be carried out to evaluate the reproductive performance of the mothers (F0 generation), behavioral studies will be carried out in the open field and in the light-dark box in PND30 (after pup weaning). The same behavioral studies will be performed with adult males. The male and female pups will be evaluated for behavior, histopathology, corticosterone, IL-1 and biochemistry using the same methodologies described for adults, at 60 days of age (PND60). Evaluation of serum corticosterone, interleukin IL-1 and biochemical studies will be held after the blood collection. For histopathological studies of the brain, liver, kidneys and testes, the organs will be removed after euthanasia of the animals. For a more detailed study of the F1 generation behavioral study, two more groups will be introduced, which will be submitted to a stress challenge by the New York subway technique. So, statistical analysis will be performed with four groups named control group (CG), stress control group (SCG), test group (TG) and stress test group (STG). One-way ANOVA followed by Tukey post-test (±=0.05%) will be performed to verify differences among means. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES, CINTHIA DOS S.; FRIAS, HUMBERTO V.; BONAMIN, LEONI V.; BONDAN, EDUARDO F.; COELHO, CIDELI P.; MARTINS, MARIA DE FATIMA M.; BERNARDI, MARIA M.; SUFFREDINI, IVANA B.. Luffa operculata (L.) Cogn. Gestational Exposition Induces Anxiety-like Behavior and Interferes with Melatonin and Inflammation in Young Female Rats. PHARMACOGNOSY RESEARCH, v. 15, n. 3, p. 13-pg., 2023-07-01. (19/12202-3, 17/03470-9)

ALVES, CINTHIA DOS S.; FRIAS, HUMBERTO V.; BONAMIN, LEONI V.; CORREIA, MICHELLE S. F.; CORRE, MONICA G.; BONDAN, EDUARDO F.; MARTINS, MARIA DE FATIMA M.; COELHO, CIDELI P.; BERNARDI, MARIA M.; SUFFREDINI, IVANA B.. Luffa operculata at a late period of gestation dysregulates melatonin and cytokines interfering with weight of dams and their male offspring. Journal of Ethnopharmacology, v. 275, p. 11-pg., 2021-04-28. (19/12202-3, 17/03470-9)

ALVES, CINTHIA DOS S.; FRIAS, HUMBERTO V.; BONAMIN, LEONI V.; CORREIA, MICHELLE S. F.; CORRE, MONICA G.; BONDAN, EDUARDO F.; MARTINS, MARIA DE FATIMA M.; COELHO, CIDELI P.; BERNARDI, MARIA M.; SUFFREDINI, IVANA B.. uffa operculata at a late period of gestation dysregulates melatonin and cytokines interfering with weight of dams and their male offsprin. Journal of Ethnopharmacology, v. 275, JUL 15 2021. (17/03470-9, 19/12202-3)

FRIAS, V, HUMBERTO; ALVES, CINTHIA DOS S.; FLORIO, JORGE C.; BONDAN, EDUARDO F.; BONAMIN, V, LEONI; COELHO, CIDELI P.; BERNARDI, MARIA M.; SUFFREDINI, IVANA B.. Vertical exposition to Luffa operculata extract deregulates behavior and hypothalamus neurotransmitters in juvenile rats. Journal of Ethnopharmacology, v. 264, JAN 10 2021. (19/12202-3, 17/03470-9)