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Cardiovascular, behavioral, neuroendocrine and morphofunctional effects evoked by acute restraint in rats: involvement of the oxytocinergic pathway

Grant number: 16/25502-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2018
Status:Discontinued
Field of knowledge:Biological Sciences - Pharmacology
Principal Investigator:Fernando Morgan de Aguiar Correa
Grantee:Ivaldo de Jesus Almeida Belém Filho
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):19/12526-3 - Effects of acute versus chronic social and non-social stress on social fear conditioning and the regulatory role of neuropeptides in male mice, BE.EP.DR

Abstract

Stress is an organism's condition in response to exposure to adverse environmental situations, involving essential adaptive responses for survival. The activation of the hypothalamus adrenal-axis, autonomic nervous system and brain structures (eg.: brainstem and amygdala), have an important role in stress, and consequently also in the related pathophysiological process. Oxytocin can be a possible treatment for disorders related to aversive situations, by modulating negative responses to stress. Thus, the aim of the present project is to evaluate cardiovascular, behavioral, endocrine and morphofunctional changes in animals exposed to acute restraint stress (RS) and its relation to the oxytocinergic pathway. Adult Wistar rats (240-260 g) will be used, and will be submitted to the RS model for 180 minutes. The participation of oxytocin in RS-evoked responses will be studied obserserving the effects of the treatment with the oxytocinergic receptor antagonist L-368,899 (3, 6 and 12 mg / kg i.p.). The mean arterial pressure, heart rate and spontaneous baroreflex sensitivity will be recorded. Twenty-four hours after the RS it will begin the behavioral evaluation on the open field test and the elevated plus maze. After each recording session, and behavioral test, it will be collected biological samples for plasma dosage of corticosterone, vasopressin and oxytocin, serum creatinine, urea, aminotransferases and creatine kinase. We also will proceed to morphological evaluation of possible alteration induced in the heart, liver, kidneys, stomach, thymus and adrenals.