Cardiovascular, behavioral, neuroendocrine and morphofunctional effects evoked by acute restraint in rats: involvement of the oxytocinergic pathway

• Grant number: 16/25502-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2018
• End date: December 31, 2021
• Field of knowledge: Biological Sciences - Pharmacology - Autonomic Pharmacology
• Principal Investigator: Fernando Morgan de Aguiar Correa
• Grantee: Ivaldo de Jesus Almeida Belém Filho
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 19/12526-3 - Effects of acute versus chronic social and non-social stress on social fear conditioning and the regulatory role of neuropeptides in male mice, BE.EP.DR

Abstract

Stress is an organism's condition in response to exposure to adverse environmental situations, involving essential adaptive responses for survival. The activation of the hypothalamus adrenal-axis, autonomic nervous system and brain structures (eg.: brainstem and amygdala), have an important role in stress, and consequently also in the related pathophysiological process. Oxytocin can be a possible treatment for disorders related to aversive situations, by modulating negative responses to stress. Thus, the aim of the present project is to evaluate cardiovascular, behavioral, endocrine and morphofunctional changes in animals exposed to acute restraint stress (RS) and its relation to the oxytocinergic pathway. Adult Wistar rats (240-260 g) will be used, and will be submitted to the RS model for 180 minutes. The participation of oxytocin in RS-evoked responses will be studied obserserving the effects of the treatment with the oxytocinergic receptor antagonist L-368,899 (3, 6 and 12 mg / kg i.p.). The mean arterial pressure, heart rate and spontaneous baroreflex sensitivity will be recorded. Twenty-four hours after the RS it will begin the behavioral evaluation on the open field test and the elevated plus maze. After each recording session, and behavioral test, it will be collected biological samples for plasma dosage of corticosterone, vasopressin and oxytocin, serum creatinine, urea, aminotransferases and creatine kinase. We also will proceed to morphological evaluation of possible alteration induced in the heart, liver, kidneys, stomach, thymus and adrenals.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA BELEM-FILHO, IVALDO JESUS; SILVA BRASIL, TAIZ FRANCINE; TRINDADE FORTALEZA, EDUARDO ALBINO; ANTUNES-RODRIGUES, JOSE; AGUIAR CORREA, FERNANDO MORGAN. functional selective effect of oxytocin secreted under restraint stress in rat. European Journal of Pharmacology, v. 904, AUG 5 2021. (16/25502-7)

BEL, IVALDO JESUS ALMEIDA; BRASIL, TAIZ FRANCINE SILVA; FORTALEZA, EDUARDO ALBINO TRINDADE; ANTUNES-RODRIGUES, JOSE; CORREA, FERNANDO MORGAN AGUIAR. A functional selective effect of oxytocin secreted under restraint stress in rats. European Journal of Pharmacology, v. 904, AUG 5 2021. (16/25502-7)

BRASIL, TAIZ F. S.; BELEM-FILHO, IVALDO J. A.; FORTALEZA, EDUARDO A. T.; ANTUNES-RODRIGUES, JOSE; CORREA, FERNANDO M. A.. The AT-1 Angiotensin Receptor is Involved in the Autonomic and Neuroendocrine Responses to Acute Restraint Stress in Male Rats. Cellular and Molecular Neurobiology, v. 42, n. 1, SI, p. 109-124, JAN 2022. (12/18556-2, 16/25502-7)

MOUSAVI, SEYEDEH MARYAM; KARIMI-HAGHIGHI, SAEIDEH; CHAVOSHINEZHAD, SARA; PANDAMOOZ, SAREH; BELEM-FILHO, IVALDO JESUS ALMEIDA; KESHAVARZ, SOMAYE; BAYAT, MAHNAZ; HOOSHMANDI, ETRAT; JABERI, ABBAS RAHIMI; SALEHI, MOHAMMAD SAIED; et al. The impacts of anesthetic regimens on the middle cerebral artery occlusion outcomes in male rats. Neuroreport, v. 33, n. 13, p. 8-pg., 2022-09-07. (19/12526-3, 16/25502-7)

ALMEIDA BELEM FILHO, IVALDO DE JESUS; SILVA BRASIL, TAIZ FRANCINE; TRINDADE FORTAZELA, EDUARDO ALBINO; RODRIGUES, JOSE ANTUNES; DE AGUIAR CORREA, FERNANDO MORGAN. Oxytocin Blocks Tachycardic Responses in the Restraint Stress Model Acting on the Oxytocin Receptor Coupled to Gq Protein. FASEB JOURNAL, v. 33, p. 3-pg., 2019-04-01. (16/25502-7)

BRASIL, TAIZ F. S.; BELEM-FILHO, IVALDO J. A.; FORTALEZA, EDUARDO A. T.; ANTUNES-RODRIGUES, JOSE; CORREA, FERNANDO M. A.. The AT-1 Angiotensin Receptor is Involved in the Autonomic and Neuroendocrine Responses to Acute Restraint Stress in Male Rats. Cellular and Molecular Neurobiology, APR 2021. (12/18556-2, 16/25502-7)

MOUSAVI, SEYEDEH MARYAM; AKBARPOUR, BIJAN; KARIMI-HAGHIGHI, SAEIDEH; PANDAMOOZ, SAREH; BELEM-FILHO, IVALDO JESUS ALMEIDA; MASIS-CALVO, MARIANELLA; SALIMI, HANIYE; LASHANIZADEGAN, RAMIN; POURAMINI, ALIREZA; OWJFARD, MARYAM; et al. Therapeutic potential of hair follicle-derived stem cell intranasal transplantation in a rat model of ischemic stroke. BMC NEUROSCIENCE, v. 23, n. 1, p. 14-pg., 2022-07-25. (19/12526-3, 16/25502-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BELÉM FILHO, Ivaldo de Jesus Almeida. Oxytocin involvement in central and peripheral effects evoked by restrain stress in rats. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.