EMU concedido no processo 2011/51295-5: image system

• Grant number: 18/07177-7
• Support Opportunities: Multi-user Equipment Program
• Start date: June 01, 2018
• End date: May 31, 2025
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Célia Regina da Silva Garcia
• Grantee: Célia Regina da Silva Garcia
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 11/51295-5 - Functional genomics in Plasmodium, AP.TEM
• EMU web page: http://www.ib.usp.br/pesquisa/caimi-equipamentos
• Type of equipment: Processos Biológicos - Caracterização - Leitores de fluorescência (imageamento, placas); Processos Biológicos - Caracterização - Leitores de placas
• Manufacturer: Nikon
• Model: Ti2-E

Abstract

This equipment will allow us to use small amounts of parasites to perform real-time studies with parasites that express GFP to decipher the molecular mechanisms of calcium signaling. These studies of cellular physiology are fundamental in our research and will also be used for screening of compounds. Plasmodium falciparum has a life cycle of 48 hours. This new system will allow us to follow this temporal process, being fundamental for studies in the different stages. Other imaging studies will be carried out, as currently we are expressing in P. falciparum a genetically encoded fluorescent marker for mitochondria. This new parasites will allow us to follow the rearrangement of this organelle during the progression of the parasite cycle. In any case, the imaging system will be available to the USP community and external users. The acquisition of this equipment will enable the training of students and Pos docs in this important area and expand collaboration with brazilian and international research groups. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, PEDRO H. S.; GARCIA, CELIA R. S.. Melatonin action in Plasmodium infection: Searching for molecules that modulate the asexual cycle as a strategy to impair the parasite cycle. Journal of Pineal Research, v. 70, n. 1, OCT 2020. (18/07177-7, 17/08684-7)

SANTOS, BENEDITO M.; DIAS, BARBARA K. M.; NAKABASHI, MYNA; GARCIA, CELIA R. S.. The Knockout for G Protein-Coupled Receptor-Like PfSR25 Increases the Susceptibility of Malaria Parasites to the Antimalarials Lumefantrine and Piperaquine but Not to Medicine for Malaria Venture Compounds. FRONTIERS IN MICROBIOLOGY, v. 12, MAR 15 2021. (18/07177-7, 17/08684-7)

DIAS, BARBARA K. M.; NAKABASHI, MYNA; ALVES, MARINA RANGEL RODRIGUES; PORTELLA, DANIELLE PAGLIAMINUTO; DOS SANTOS, BENEDITO MATHEUS; ALMEIDA, FAHYME COSTA DA SILVA; RIBEIRO, RAMIRA YURI; SCHUCK, DESIREE C.; JORDAO, ALESSANDRO KAPPEL; GARCIA, CELIA R. S.. The Plasmodium falciparum eIK1 kinase (PfeIK1) is central for melatonin synchronization in the human malaria parasite. Melatotosil blocks melatonin action on parasite cell cycle. Journal of Pineal Research, v. 69, n. 3, p. 17-pg., 2020-08-07. (18/07177-7, 17/08684-7)

PEREIRA, PEDRO H. S.; GARCIA, CELIA R. S.. Evidence of G-Protein-Coupled Receptors (GPCR) in the Parasitic Protozoa Plasmodium falciparum-Sensing the Host Environment and Coupling within Its Molecular Signaling Toolkit. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 22, NOV 2021. (17/08684-7, 18/07177-7)

SINGH, MANEESH K.; TESSARIN-ALMEIDA, GIULLIANA; DIAS, BARBARA K. M.; PEREIRA, PEDRO SCARPELLLI; COSTA, FAHYME; PRZYBORSKI, JUDE M.; GARCIA, CELIA R. S.. A nuclear protein, PfMORC confers melatonin dependent synchrony of the human malaria parasite P. falciparum in the asexual stage. SCIENTIFIC REPORTS, v. 11, n. 1, JAN 21 2021. (17/08684-7, 19/09490-7, 11/51295-5, 18/07177-7)

ALVES, EDUARDO; NAKAYA, HELDER; GUIMARES, EUZEBIO; GARCIA, CELIA R. S.. Combining IP3 affinity chromatography and bioinformatics reveals a novel protein-IP3 binding site on Plasmodium falciparum MDR1 transporter*. CURRENT RESEARCH IN MICROBIAL SCIENCES, v. 4, p. 7-pg., 2022-12-19. (17/08684-7, 18/14933-2, 18/07177-7)

BORGES-PEREIRA, LUCAS; DIAS, BARBARA K. M.; SINGH, MANEESH KUMAR; GARCIA, CELIA R. S.. Malaria parasites and circadian rhythm: New insights into an old puzzle. CURRENT RESEARCH IN MICROBIAL SCIENCES, v. 2, p. 3-pg., 2021-12-01. (17/08684-7, 18/07177-7, 16/14411-0, 19/09490-7)

DIAS, BARBARA K. M.; NAKABASHI, MYNA; ALVES, MARINA RANGEL RODRIGUES; PORTELLA, DANIELLE PAGLIAMINUTO; DOS SANTOS, BENEDITO MATHEUS; ALMEIDA, FAHYME COSTA DA SILVA; RIBEIRO, RAMIRA YURI; SCHUCK, DESIREE C.; JORDAO, ALESSANDRO KAPPEL; GARCIA, CELIA R. S.. ThePlasmodium falciparumeIK1 kinase (PfeIK1) is central for melatonin synchronization in the human malaria parasite. Melatotosil blocks melatonin action on parasite cell cycle. Journal of Pineal Research, v. 69, n. 3, AUG 2020. (17/08684-7, 18/07177-7)

BORGES-PEREIRA, LUCAS; THOMAS, SAMANTHA J.; DOS ANJOS E SILVA, AMANDA LAIZY; BARTLETT, PAULA J.; THOMAS, ANDREW P.; GARCIA, CELIA R. S.. The genetic Ca2+ sensor GCaMP3 reveals multiple Ca2+ stores differentially coupled to Ca2+ entry in the human malaria parasite Plasmodium falciparum. Journal of Biological Chemistry, v. 295, n. 44, p. 14998-15012, OCT 30 2020. (18/07177-7, 16/14411-0, 17/08684-7)

SCARPELLI, PEDRO H.; PECENIN, MATEUS F.; GARCIA, CELIA R. S.. Intracellular Ca2+ Signaling in Protozoan Parasites: An Overview with a Focus on Mitochondria. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 1, JAN 2021. (17/08684-7, 18/07177-7)

DEDA, DAIANA K.; IGLESIAS, BERNARDO A.; ALVES, EDUARDO; ARAKI, KOITI; GARCIA, CELIA R. S.. Porphyrin Derivative Nanoformulations for Therapy and Antiparasitic Agents. Molecules, v. 25, n. 9, MAY 2020. (17/08684-7, 18/07177-7, 18/21489-1)