Scientific, technological and infrastructure qualification in radiopharmaceuticals, radiation and entrepreneurship for health purposes (PDIp)

• Grant number: 17/50332-0
• Support Opportunities: Research Grants - State Research Institutes Modernization Program
• Duration: April 01, 2018 - March 31, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal Investigator: Marcelo Linardi
• Grantee: Marcelo Linardi
• Host Institution: Instituto de Pesquisas Energéticas e Nucleares (IPEN). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São Paulo , SP, Brazil
• Pesquisadores principais: Ademar Benévolo Lugão ; Artur Wilson Carbonari ; Elaine Bortoleti de Araújo ; Maria Elisa Chuery Martins Rostelato ; Monica Beatriz Mathor ; Niklaus Ursus Wetter ; Nilson Dias Vieira Junior
• Associated grant(s): 18/25754-1 - Multi user equipment approved in grant FAPESP 2017/50332-0: digital mammograph, AP.EMU; 19/24085-1 - Development and characterization of nanoparticulate biomaterials for therapeutic and diagnostic applications in medicine, AV.EXT; 19/17154-7 - Development and characterization of nanoparticulated biomaterials for medicine therapy and diagnostic applications, AV.EXT; + associated grants 18/25961-7 - Laser particle acceleration and applications, AP.R SPRINT; 18/19251-7 - Multi-User Equipment approved in grant 2017/50332-0: ASAP 2020 plus surface area analyzer, volume and pore area, AP.EMU; 18/19240-5 - Multi-user equipment approved in grant 2017-50332-0: STM-AFM Raman SNOM analysis system, AP.EMU - associated grants
• Associated scholarship(s): 22/07199-6 - Characterization of nanocomposites and their interaction with cellular tissue by SNOM techniques for the purpose of new drugs, BP.PD; 21/13737-8 - Acceleration of laser particles in the Scientific, Technological and Infrastructure Training program in Radiopharmaceuticals and Entrepreneurship at the Service of Health., BP.PD; 19/15154-0 - Smart immunotherapeutic gold and palladium nanoparticles functionalized with immunomodulatory phytochemicals (mangiferin, resveratrol, piceatanol, naringin, silibilin, and astringin) and their use as radiosensitizers for tumor ablation therapy, BP.PD; + associated scholarships 19/15620-0 - Tuning MFe2O4 (M=Co,Ni) nanoparticles for in vivo biomedical applications: Doping with rare earths and biocompatible coating, BP.PD; 19/15640-1 - The role of Tissue Engineering on the development of in vitro models for biocompatibility tests and biological assessment, BP.PD; 18/18526-2 - Radioactive Nanosource for Use in Brachytherapy, BP.PD; 19/10110-4 - Studies of adequacy of radiolabeling of PSMA-1007 with fluorine-18 : validation of the analytical methodology and pre-clinical evaluation in the diagnosis of prostate cancer, BP.MS; 19/04172-7 - Pharmacotechnical development of DOTATATO lyophilized kit for 68Ga labeling and preclinical evaluation, BP.DR; 18/18928-3 - Development and Application of Theranostic Biomaterials: Evaluation of Cellular Response to Fluorescent Nanoparticles of Lanthanide and Magnesium-doped Calcium Phosphates and its Applicability as Non-Viral Vectors for Gene Therapy, BE.PQ; 18/12965-4 - Obtaining and evaluating the applicability in the treatment of Prostate Cancer of the PSMA-617 kit for prompt radiolabeling with lutetium-177, BP.DR; 18/13096-0 - Study for implantation of the radiopharmaceutical FES-18F process production in an automated system: process validation and analytical methods approach, BP.MS - associated scholarships

Abstract

The Scientific and Technological Qualification refers to the necessary adaptation to the development of new radiopharmaceuticals, specifically in the phase of scheduling until pilot, within the required quality standards. In addition, one must consider safety issues for manipulation of high radioactive activities, compatible with the scale of industrial production, making it possible to close development cycle for new products, destined to conduct clinical studies in order to demonstrate their efficacy and safety. In addition, this capacitation includes the development of advanced laboratory practices for the chemical and isotopic qualification of radiopharmaceuticals and nanobiomaterials, for health application, via mass spectrometry technique, among others. Radiopharmaceuticals produced at IPEN need assessment by pre-clinical tests for ANVISA approval. This project also aims to carry out research to evaluate the cytotoxic and genotoxic activity of new radiopharmaceutical candidates, as well as to improve radiation detection systems and the processes associated with radiotherapy, nuclear medicine and radio diagnosis, using high performance computing. Considering the qualification in R&D Infrastructure, the project aims to create a nucleus of imaging studies in molecules, cells, tissues and nanoparticles associated to biological processes at IPEN, unprecedented in the country, by SNOM and micro-FTIR, for innovative use in medicine. The proposed techniques will allow mapping of ultrastructural and cellular changes before and after a given treatment, by either laser, nanoparticules, brachytherapy, or radiopharmaceutical. On another front of R&D, the objective is to demonstrate the feasibility of the acceleration of charged particles (electrons/protons/hadrons) in compact systems with lower cost, compared to conventional technologies of acceleration, aiming to proton therapy for nuclear medicine. Closing the virtuous cycle of Innovation and Entrepreneurship, the project aims to improve the management of knowledge and intellectual property generated at IPEN and its interface with the productive sector, in specific actions. (AU)

Scientific publications

(19)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VILLANI, D.; RODRIGUES, JR., O.; CAMPOS, L. L.. Dosimetric characterization of 3D printed phantoms at different infill percentages for diagnostic X-ray energy range. Radiation Physics and Chemistry, v. 172, JUL 2020. (17/50332-0)

LOPES, MNICA SCHAFFER; PEREIRA, DAISA LIMA; BRAINER DE OLIVEIRA MOTA, CLAUDIA CRISTINA; AMARAL, MARCELLO MAGRI; ZEZELL, DENISE MARIA; LEONIDAS GOMES, ANDERSON STEVENS. The lingual enamel morphology and bracket shear bond strength influenced by Nd:YAG laser and aluminum oxide sandblasting preconditioning. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 3, JUN 2020. (17/50332-0, 05/51689-2)

SANTOS, V, RENATA; CABRERA-PASCA, GABRIEL A.; COSTA, CLEIDILANE S.; BOSCH-SANTOS, BRIANNA; OTUBO, LARISSA; PEREIRA, LUCIANO F. D.; CORREA, BRUNO S.; EFFENBERGER, FERNANDO B.; BURIMOVA, ANASTASIA; FREITAS, RAFAEL S.; et al. Crystalline and magnetic properties of CoO nanoparticles locally investigated by using radioactive indium tracer. SCIENTIFIC REPORTS, v. 11, n. 1, OCT 25 2021. (19/15620-0, 17/50332-0, 15/16191-5)

CORREA, BRUNO S.; COSTA, MESSIAS S.; CABRERA-PASCA, GABRIEL A.; SENA, CLEIDILANE; PINTO, RAFAEL H.; SILVA, ANA PAULA S.; CARVALHO JUNIOR, RAUL N.; ISHIDA, LINA; RAMON, JONATHAN G. A.; FREITAS, RAFAEL S.; et al. High-saturation magnetization in small nanoparticles of Fe3O4 coated with natural oils. JOURNAL OF NANOPARTICLE RESEARCH, v. 22, n. 3, MAR 6 2020. (15/16191-5, 17/50332-0)

SILVA, FELIPE D.; OLIVEIRA, JOAO E.; FREIRE, RENAN P.; SUZUKI, MIRIAM F.; SOARES, CARLOS R.; BARTOLINI, PAOLO. Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis. AMB EXPRESS, v. 9, n. 1, AUG 29 2019. (15/26058-0, 17/50332-0)

ZANINI, NATHALIA A.; RABELO, THAIS F.; ZAMATARO, CLAUDIA B.; CARAMEL-JUVINO, AMANDA; ANA, PATRICIA A.; ZEZELL, DENISE M.. Morphological, optical, and elemental analysis of dental enamel after debonding laminate veneer with Er,Cr:YSGGlaser: A pilot study. MICROSCOPY RESEARCH AND TECHNIQUE, v. 84, n. 3, p. 489-498, MAR 2021. (17/50332-0, 05/51689-2)

VINÍCIUS MAXIMIANO; ALANA CRISTINA MACHADO; RAQUEL MARIANNA LOPES; FERNANDA ELLEN MERCATELLI RABELO; STEPHANIE ASSIMAKOPOULOS GARÓFALO; DENISE MARIA ZEZELL; ANA CECILIA CORRÊA ARANHA; TAÍS SCARAMUCCI. Association of Nd:YAG laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion. Journal of Applied Oral Science, v. 29, 2021-03-31. (17/02341-0, 17/24714-3, 17/50332-0)

BOSCH-SANTOS, B.; NASCIMENTO, N. M.; SAIKI, M.; CORREA, E. L.; SALES, T. S. N.; PEREIRA, L. F. D.; CABRERA-PASCA, G. A.; SAXENA, R. N.; SCHELL, J.; CARBONARI, A. W.. Magnetic field at Ce impurities in La sites of La0.5Ba0.5MnO3 double perovskites. AIP ADVANCES, v. 9, n. 3, MAR 2019. (17/50332-0)

DE CARVALHO, L. R.; VIEIRA, D. P.. Evaluation of genotoxic potential of peptides used in nuclear medicine (PSMA -617 and -11, and ubiquicidine 29-41) using a flow-cytometric, semi-automated analysis of micronuclei frequency in cell cultures. TOXICOLOGY REPORTS, v. 7, p. 304-316, 2020. (17/50332-0)

DIAS-MORAES, MARCIA CRISTINA; CASTRO, PEDRO ARTHUR AUGUSTO; PEREIRA, DAISA LIMA; ANA, PATRICIA APARECIDA; FREITAS, ANDERSON ZANARDI; ZEZELL, DENISE MARIA. Assessment of the preventive effects of Nd:YAG laser associated with fluoride on enamel caries using optical coherence tomography and FTIR spectroscopy. PLoS One, v. 16, n. 7, JUL 7 2021. (17/50332-0, 05/51689-2)

CORREA, E. L.; BOSCH-SANTOS, B.; SAXENA, R. N.; CABRERA-PASCA, G. A.; CARBONARI, A. W.. Effects of an external magnetic field on the hyperfine parameters in RE2O3 (RE = Gd, Er) nanoparticles measured by perturbed angular correlation spectroscopy. AIP ADVANCES, v. 10, n. 1, JAN 2020. (17/50332-0)

DARUICH DE SOUZA, CARLA; BUENO BARBEZAN, ANGELICA; ARCOS ROSERO, WILMMER ALEXANDER; NASCIMENTO DOS SANTOS, SOFIA; VERGACAS DE SOUSA CARVALHO, DIEGO; ZEITUNI, CARLOS ALBERTO; SOARES BERNARDES, EMERSON; PEREZ VIEIRA, DANIEL; SPENCER, PATRICK JACK; SIMOES RIBEIRO, MARTHA; et al. ynthesis, In Vitro Testing, and Biodistribution of Surfactant-Free Radioactive Nanoparticles for Cancer Treatmen. NANOMATERIALS, v. 12, n. 2, JAN 2022. (18/18526-2, 17/50332-0)

SALES, T. S. N.; BURIMOVA, A.; RODRIGUES, P. S.; MATOS, I. T.; CABRERA-PASCA, G. A.; SAXENA, R. N.; PEREIRA, L. F. D.; OTUBO, L.; CARBONARI, A. W.. Synthesis and characterization of Fe3O4-HfO2 nanoparticles by hyperfine interactions measurements. AIP ADVANCES, v. 11, n. 1, JAN 1 2021. (19/15620-0, 17/50332-0)

AMARAL, MARCELLO M.; ZEZELL, DENISE M.; MONTE, ADAMO F. G.; DE CARA, ANA C. B.; ARAUJO, JEANN C. R.; ANTUNES, ANDREA; FREITAS, ANDERSON Z.. General model for depth-resolved estimation of the optical attenuation coefficients in optical coherence tomography. Journal of Biophotonics, v. 12, n. 10, JULY 2019. (10/03123-8, 17/50332-0, 05/51689-2)

SAVI, MATHEUS; ANDRADE, MARCO A. B.; POTIENS, MARIA P. A.. Commercial filament testing for use in 3D printed phantoms. Radiation Physics and Chemistry, v. 174, SEP 2020. (17/50332-0)

SUZUKI, MIRIAM F.; ALMEIDA, LARISSA A.; POMIN, STEPHANIE A.; SILVA, FELIPE D.; FREIRE, RENAN P.; OLIVEIRA, JOAO E.; AFFONSO, REGINA; SOARES, CARLOS R. J.; BARTOLINI, PAOLO. Periplasmic synthesis and purification of the human prolactin antagonist Delta(1-11)-G129R-hPRL. AMB EXPRESS, v. 11, n. 1, APR 27 2021. (12/24345-4, 17/50332-0, 15/26058-0)

CASTRO, PEDRO A. A.; LIMA, CASSIO A.; MORAIS, MYCHEL R. P. T.; ZORN, TELMA M. T.; ZEZELL, DENISE M.. Monitoring the Progress and Healing Status of Burn Wounds Using Infrared Spectroscopy. Applied Spectroscopy, v. 74, n. 7, MAY 2020. (13/26113-6, 17/50332-0, 05/51689-2)

DEL-VALLE, MATHEUS; SANTOS, MOISES OLIVEIRA DOS; SANTOS, SOFIA NASCIMENTO DOS; CASTRO, PEDRO ARTHUR AUGUSTO DE; BERNARDES, EMERSON SOARES; ZEZELL, DENISE MARIA. The impact of scan number and its preprocessing in micro-FTIR imaging when applying machine learning for breast cancer subtypes classification. VIBRATIONAL SPECTROSCOPY, v. 117, NOV 2021. (17/50332-0)

CAMPOS, ALEX C.; PAES, SUANE C.; CORREA, BRUNO S.; CABRERA-PASCA, GABRIEL A.; COSTA, MESSIAS S.; COSTA, CLEIDILANE S.; OTUBO, LARISSA; CARBONARI, A. W.. Growth of Long ZnO Nanowires with High Density on the ZnO Surface for Gas Sensors. ACS APPLIED NANO MATERIALS, v. 3, n. 1, p. 175-185, JAN 2020. (17/50332-0)