Evaluation of offspring exposed to mildly diabetic intrauterine millieu, submitted to postnatal hyperlipid diet and treated with a mixture of calcium and vitamin D during pregnancy.

• Grant number: 16/25207-5
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2018
• End date: August 31, 2020
• Field of knowledge: Health Sciences - Nutrition - Dietetics
• Principal Investigator: Débora Cristina Damasceno
• Grantee: Débora Cristina Damasceno
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Gustavo Tadeu Volpato ; Yuri Karen Sinzato

Abstract

Diabetes is a syndrome characterized by a functional change in the beta-pancreatic cell in insulin synthesis/secretion or due to an inadequate insulin action in peripheral tissues, leading to increased blood glucose concentration (hyperglycemia). The literature describes the pathophysiological mechanisms, such as oxidative stress, are involved in the repercussions of hyperglycemia in various organs and systems. Pregnancy alone causes an imbalance between free radical production and antioxidant defense system, causing oxidative stress. Due to study variables and for ethical reasons, many experimental models are used to investigate diabetes and pregnancy association. In our research laboratory, a number of models for the induction of severe diabetes (mimicking the glycemia of decompensated type 1 Diabetes mellitus) and of mild intensity (to reproduce the glycemia of DM type 2 and / or Gestational DM) were developed. These models were relevant to improve our understanding of the mechanisms in the diabetes and pregnancy in rats, especially considering the repercussions in the maternal organism (reproductive performance, hormonal analyses in the endocrine pancreas, oxidative stress and lipid profile in blood and liver), fetal growth and development and morphological evaluation, cytokine levels and oxidative stress in the placenta. In view of these results, there is evidence to confirm the fetal programming due to an unfavorable maternal intrauterine environment caused by severe diabetes and even by mild diabetes. Based on the fact that offspring born to mildly diabetic rats present altered glycemic changes in adulthood and impaired reproductive performance, and considering a parallelism between the human condiction and experimental related to children born to diabetic mothers to feed inadequately after the maternal breast-feeding during postnatal life, we objectived to evaluate the linking between these two unfavorable condictions (hyperglycemic intrauterine environment and postnatal hyperlipidic diet) in relation to reproductive parameters, oxidative stress, endocrine pancreas, lipid profile, fetal and perinatal growth. In addition, as previous results in our laboratory confirmed the beneficial effects of a calcium and vitamin D mixture in mildly diabetic rats we will also investigate how the treatment using this mixture influences offspring from diabetic rats exposed to the hyperlipid diet from weaning. This study aims at normalizing the redox state of these rats, contributing to a better condiction of the maternal organs and systems and favoring fetal programming. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARAUJO-SILVA, VANESSA CARULINE; SANTOS-SILVA, ALICE; LOURENCO, ANDRESSA SILVA; BARROS-BARBOSA, CRISTIELLY MARIA; MORAES-SOUZA, RAFAIANNE QUEIROZ; SOARES, THAIGRA SOUSA; KARKI, BARSHANA; PAULA, VERONYCA GONCALVES; SINZATO, YURI KAREN; DAMASCENO, DEBORA CRISTINA; et al. Congenital Anomalies Programmed by Maternal Diabetes and Obesity on Offspring of Rats. FRONTIERS IN PHYSIOLOGY, v. 12, AUG 10 2021. (16/25207-5)

SAULLO, CAROLINA M.; SINZATO, YURI K.; PAULA, VERONYCA G.; GALLEGO, FRANCIANE Q.; CORRENTE, JOSE E.; IESSI, ISABELA L.; VOLPATO, GUSTAVO T.; DAMASCENO, DEBORA C.. Exposure to maternal hyperglycemia and high-fat diet consumption after weaning in rats: repercussions on periovarian adipose tissue. JOURNAL OF DEVELOPMENTAL ORIGINS OF HEALTH AND DISEASE, DEC 2021. (16/25207-5)

PAULA, VERONYCA G.; VESENTINI, GIOVANA; SINZATO, YURI K.; MORAES-SOUZA, RAFAIANNE Q.; VOLPATO, GUSTAVO T.; DAMASCENO, DEBORA C.. Intergenerational high-fat diet impairs ovarian follicular development in rodents: a systematic review and meta-analysis. NUTRITION REVIEWS, v. 80, n. 4, p. 15-pg., 2021-08-30. (16/25207-5)

SAULLO, CAROLINA; DA CRUZ, LARISSA LOPES; DAMASCENO, DEBORA CRISTINA; VOLPATO, GUSTAVO TADEU; SINZATO, YURI KAREN; KARKI, BARSHANA; GALLEGO, FRANCIANE QUINTANILHA; VESENTINI, GIOVANA. Effects of a maternal high-fat diet on adipose tissue in murine offspring: A systematic review and meta-analysis. Biochimie, v. 201, p. 15-pg., 2022-07-06. (16/25207-5)

SINZATO, YURI K.; RODRIGUES, THIAGO; CRUZ, LARISSA L.; BARCO, VINICIUS S.; SOUZA, MAYSA R.; VOLPATO, GUSTAVO T.; DAMASCENO, DEBORA C.. Assessment of Oxidative Stress Biomarkers in Rat Blood. BIO-PROTOCOL, v. 13, n. 5, p. 26-pg., 2023-03-05. (16/25207-5)

PAULA, VERONYCA GONCALVES; DE SOUZA, MAYSA ROCHA; SINZATO, YURI KAREN; SILVA BALBIN VILLAVERDE, ANA IZABEL; CORRENTE, JOSE EDUARDO; VOLPATO, GUSTAVO TADEU; DAMASCENO, DEBORA CRISTINA. Nonpregnant and pregnant adult female rats affected by maternal diabetes environment. SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE, v. 68, n. 5-6, p. 12-pg., 2022-09-14. (16/25207-5)

DA CRUZ, LARISSA LOPES; VESENTINI, GIOVANA; SINZATO, YURI KAREN; VILLAVERDE, ANA IZABEL SILVA BALBIN; VOLPATO, GUSTAVO TADEU; DAMASCENO, DEBORA CRISTINA. Effects of high-fat diet-induced diabetes on autophagy in the murine liver: A systematic review and meta-analysis. Life Sciences, v. 309, p. 13-pg., 2022-10-02. (16/25207-5)

MARIA EDUARDA P. GOMES; LUIGI M.J. DIDOMIZIO; YURI K. SINZATO; VERÔNYCA G. PAULA; MAYSA R. SOUZA; FRANCIANE Q. GALLEGO; VINÍCIUS S. BARCO; GUSTAVO T. VOLPATO; DÉBORA CRISTINA DAMASCENO. Influence of maternal periuterine and periovarian fat on reproductive performance and fetal growth in rats. Anais da Academia Brasileira de Ciências, v. 95, 2023-12-04. (16/25207-5)

PAULA, VERONYCA G.; SINZATO, YURI K.; SOUZA, RAFAIANNE Q. M.; CRUZ, LARISSA L.; KLOPPEL, EDUARDO; GALLEGO-SOUZA, FRANCIANE Q.; BARCO, VINICIUS S.; SAULLO, CAROLINA B.; VESENTINI, GIOVANA; VOLPATO, GUSTAVO T.; et al. Repercussion of Maternal Diabetes and Post-Weaning High-Fat Diet Consumption in Laboratory Animals. METABOLISM-CLINICAL AND EXPERIMENTAL, v. 116, p. 1-pg., 2021-01-29. (16/25207-5)

VINÍCIUS S. BARCO; FRANCIANE Q. GALLEGO; VERÔNYCA G. PAULA; LARISSA L. CRUZ; BARSHANA KARKI; GUSTAVO T. VOLPATO; DÉBORA C. DAMASCENO. Effects of diabetes between generations on the pre-embryos of rats. Anais da Academia Brasileira de Ciências, v. 94, 2022-12-12. (16/25207-5)

PAULA, VERONYCA GONCALVES; SINZATO, YURI KAREN; DE MORAES-SOUZA, RAFAIANNE QUEIROZ; SOARES, THAIGRA SOUSA; GALLEGO SOUZA, FRANCIANE QUINTANILHA; KARKI, BARSHANA; DE ANDRADE PAES, ANTONIO MARCUS; CORRENTE, JOSE EDUARDO; DAMASCENO, DEBORA CRISTINA; VOLPATO, GUSTAVO TADEU. etabolic changes in female rats exposed to intrauterine hyperglycemia and postweaning consumption of high-fat die. BIOLOGY OF REPRODUCTION, v. 106, n. 1, p. 200-212, JAN 2022. (16/25207-5)

SINZATO, YURI KAREN; PAULA, VERONYCA GONCALVES; GALLEGO, FRANCIANE QUINTANILHA; MORAES-SOUZA, RAFAIANNE Q.; CORRENTE, JOSE EDUARDO; VOLPATO, GUSTAVO TADEU; DAMASCENO, DEBORA CRISTINA. Maternal Diabetes and Postnatal High-Fat Diet on Pregnant Offspring. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v. 10, p. 15-pg., 2022-05-30. (16/25207-5)

KLOPPEL, EDUARDO; SINZATO, YURI K.; RODRIGUES, TIAGO; GALLEGO, FRANCIANE Q.; KARKI, BARSHANA; VOLPATO, GUSTAVO T.; CORRENTE, JOSE E.; ROY, SAYON; DAMASCENO, DEBORA C.. Benefits of Vitamin D Supplementation on Pregnancy of Rats with Pregestational Diabetes and Their Offspring. REPRODUCTIVE SCIENCES, v. N/A, p. 16-pg., 2022-08-23. (16/25207-5)