ANTIBIO-BAC: exploring the bacterial cell wall as a target for novel antibiotherapies
| Grant number: | 17/50451-0 |
| Support type: | Regular Research Grants |
| Duration: | August 01, 2018 - July 31, 2019 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine |
| Cooperation agreement: | BBSRC, UKRI ; Newton Fund, with FAPESP as a partner institution in Brazil |
| Principal Investigator: | Helder Louvandini |
| Grantee: | Helder Louvandini |
| Principal investigator abroad: | Caroline Rymer |
| Institution abroad: | University of Reading, England |
| Home Institution: | Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil |
Abstract
The hypothesis of this trial is to verify if there are differences associated with system and agro ecological zone in terms of the prevalence of AMR. Dietary and management interventions (such as tannins) might alter the microbiome of the host animal to alter its level of AMR. To test this, we would sample faeces from beef animals of different ages from a range of different systems and agro ecological zones. The faecal samples will be analyzed for functional metagenomics of AMR (at CENA), microbiome composition by 168 RNA (at UoR) and fecal culturing to determine phenotypic AMR (at CENA). Forage samples would be taken for analysis of chemical composition and secondary plant metabolites/bioactive compounds by NMR (at UoR). From those data, we will determine which agro ecological zones/systems would be associated with high or low prevalence of AMR. In the second phase, in more controlled studies, we would then aim to alter the microbiome of the high AMR animals to mirror those of the low AMR animals. At this point, we would also investigate the range of AMR associated with soil microbiome in different regions. (AU)