Next-Generation sequencing for study of Feline Immunodeficiency virus (FIV) complete genome and viral quasispecies analysis

• Grant number: 17/24907-6
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2018
• End date: May 31, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Sueli Akemi Taniwaki Miyagi
• Grantee: Sueli Akemi Taniwaki Miyagi
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: João Pessoa Araújo Junior ; Leonardo José Richtzenhain ; Paulo Eduardo Brandão ; Taís Fukuta da Cruz

Abstract

Feline immunodeficiency virus (FIV) infection is the one of most important infectious diseases of domestic cats. For this reason, and because it causes an acquired immunodeficiency syndrome (AIDS) similar to Human immunodeficiency virus (HIV) in humans, FIV has been widely studied. Nevertheless, there are only a few descriptions of FIV complete genome and viral quasispecies analysis, and none using Next-Generation sequencing (NGS). Based on this, the present project aims to use NGS to obtain FIV genome sequences and to perform viral quasispecies analysis. The coding region plus the 3' untranslated region (U3) of FIV genome will be amplified from proviral DNA of naturally infected cats. The amplicons will be submitted to NGS on MiSeq system (Illumina) and the sequences will be used to make de novo assembly of nearly complete FIV genome and viral quasispecies analysis. Until this moment, there is no description of FIV complete genome of domestic cats from Brazil, and even on GenBank only 7 sequences are available. Therefore, this project expected to standardize a method for amplification and sequencing of FIV genome in a simple way, allowing a broader analysis to increase the knowledge on the biology and evolution of the virus and virus-host interaction. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TANIWAKI, SUELI A.; JIMENEZ-VILLEGAS, TATIANA; SANTANA-CLAVIJO, NELSON F.; CRUZ, TAIS F.; SILVA, SHEILA O. S.; VALENCIA-BACCA, JUAN D.; LOAIZA-PEDREROS, FELIPE; RICHTZENHAIN, LEONARDO J.; FERREIRA, FERNANDO; ARAUJO JUNIOR, JOAO P.; et al. Near-Complete Genome Sequence of Feline Immunodeficiency Virus from Colombia. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 9, n. 33, AUG 2020. (16/09518-0, 17/24907-6)