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Jaboticaba Extract: In vivo response of prostatic adenocarcinoma in TRAMP mice associated with hyperlipic diet intake and in vitro response of tumoral human prostatic cells

Abstract

Prostate cancer (CaP) is the most diagnosed type of cancer in human beings. Literature has correlated hypelipic diet intake with prostate cancer progression. In addition to this, it is known that antioxidants, such as polyphenols, have been evaluated in cancer therapies. The Jaboticaba, a natural antioxidant, is being studied and shows the antiproliferative and antioxidant effects on the different cancer lineage cells of the prostate. However, only few studies have related Jaboticaba ingestion with cancer protection, as well as with different signaling in the prostatic microenvironment, which could be activated with Jaboticaba administration. The aim of the study herein is the evaluation of metabolic, structural, oxidative, inflammatory and molecular parameters in the prostatic microenvironment from TRAMP mice submitted to the administration of Jaboticaba peel extract from 8 to 16 weeks of age and submitted or not to a hyperlipidic diet intake. In addition, we aim to evaluate the in vitro PC-3 and LNCaP cellular response treated with Jaboticaba peel extract, characterizing genes involved in the inflammatory pathway by means of PCR array. For the in vivo evaluation, we have the following experimental groups: control group (C8)-8 week old TRAMP mice; control group normolipidic diet (C16)- 8 week old TRAMP mice; Jaboticaba group normolipidic diet (JC)- 8 week old TRAMP mice which will receive five Jaboticaba doses (0.007 mL/ g/animal/day); control hyperlipidic diet (CH16)-8 week old TRAMP mice which will receive hiperlipidic diet; Jaboticaba group hyperlipidic diet (JH)- 8 week old TRAMP mice which will receive hyperlipidic diet and five Jaboticaba doses (0.007 mL/ g/animal/day). All the mice from all the different groups will be sacrificed at 16 weeks of age. At the end of treatment, the dorsolateral lobes and the plasma of the TRAMP mice will be collected for structural, immunohistochemistry, Western Blotting, Elisa, RT-PCR and hybridization in situ evaluations. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NOGUEIRA-LIMA, ELLEN; LAMAS, CELINA DE ALMEIDA; BASEGGIO, ANDRESSA MARA; FERNANDES DO VALE, JESSICA STEPHANY; MAROSTICA JUNIOR, MARIO ROBERTO; ALVES CAGNON, VALERIA HELENA. High-fat diet effects on the prostatic adenocarcinoma model and jaboticaba peel extract intake: protective response in metabolic disorders and liver histopathology. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, v. 72, n. 8 NOV 2019. Web of Science Citations: 2.

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