Role of sCD100 in in vitro and in vivo infection by different species of Leishmania

• Grant number: 18/14972-8
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2018
• End date: May 31, 2021
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Beatriz Simonsen Stolf
• Grantee: Beatriz Simonsen Stolf
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Leishmaniasis is a zoonotic disease caused by protozoa of the genus Leishmania that may be classified in tegumentary (TL) or visceral (VL) forms. In humans and other vertebrate hosts, Leishmania preferentially infects macrophages. Many factors affect the form and severity of the disease, mainly the species of Leishmania and the host immune response. CD100 (Sema 4D) is a glycoprotein of the semaphoring family, which plays essential roles in the immune system. Our group showed that the soluble recombinant CD100 (sCD100) increases the infection of murine macrophages by Leishmania (Leishmania) amazonensis by interacting with CD72 receptor. We also showed increase in zymosan phagocytosis and in Trypanosoma cruzi infection. We believe similar increases in infection will be observed with other Leishmania species. Therefore, one of the aims of this project is to evaluate the effect of sCD100 on in vitro infection and in phagocytosis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi, the species most commonly involved in TL and VL in Brazil, respectively. We will also investigate the effects of sCD100 on the infection of human macrophages. The mechanisms underlying the increase in infectivity of Leishmania will be determined by the proteomic analysis of proteins differentially expressed in macrophages treated with sCD100. The role of CD100 in vivo will be evaluated by comparing the infectivity of L. (L.) amazonensis in wild type mice and mice knockout for CD100. We believe that this study will help to elucidate the mechanisms by which CD100 increases the infectivity of Leishmania in macrophages and the role of this molecule in leishmaniasis. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARBOSA, GUSTAVO ROLIM; MARANA, SANDRO ROBERTO; STOLF, BEATRIZ SIMONSEN. Characterization of Leishmania (L.) amazonensis oligopeptidase B and its role in macrophage infection. Parasitology, v. N/A, p. 8-pg., 2022-06-15. (18/14972-8, 19/02391-3)

CAVALCANTE, TAINA; MEDEIROS, MARIANA MEDINA; MULE, SIMON NGAO; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. The Role of Sialic Acids in the Establishment of Infections by Pathogens, With Special Focus on Leishmania. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, MAY 13 2021. (17/04032-5, 18/18257-1, 18/15549-1, 18/14972-8)

TANO, FABIA TOMIE; TELLERIA, ERICH LOZA; REGO, FELIPE DUTRA; COELHO, FELIPE SOARES; DE REZENDE, ELOIZA; SOARES, RODRIGO PEDRO; TRAUB-CSEKO, YARA MARIA; STOLF, BEATRIZ SIMONSEN. Phenotypical Differences between Leishmania (Leishmania) amazonensis PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies. PATHOGENS, v. 12, n. 2, p. 13-pg., 2023-02-01. (18/14972-8, 21/08915-4, 17/26197-6)

TANO, FABIA TOMIE; BARBOSA, GUSTAVO ROLIM; DE REZENDE, ELOIZA; OLIVEIRA SOUZA, RODOLPHO ORNITZ; MUXEL, SANDRA MARCIA; SILBER, ARIEL MARIANO; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. Proteome and morphological analysis show unexpected differences between promastigotes of Leishmania amazonensis PH8 and LV79 strains. PLoS One, v. 17, n. 8, p. 26-pg., 2022-08-23. (18/15549-1, 18/18257-1, 18/14972-8, 17/26197-6, 14/06863-3)

GUILHERME MOREIRA PAIVA CARRARA; BEATRIZ SIMONSEN STOLF. FVB/NJ strain as a mouse model for cutaneous leishmaniasis by Leishmania (L.) amazonensis. Memórias do Instituto Oswaldo Cruz, v. 119, 2024-03-15. (21/08915-4, 18/14972-8)