Impact of inspiratory muscle training and aging on metabolic mapping, autonomic modulation, and cardiovascular, respiratory and metabolic responses, and prediction of cardiorespiratory health through wearables

• Grant number: 16/22215-7
• Support Opportunities: Research Projects - Thematic Grants
• Start date: October 01, 2018
• End date: September 30, 2024
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Aparecida Maria Catai
• Grantee: Aparecida Maria Catai
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• City of the host institution: São Carlos
• Principal investigators: Antonio Gilberto Ferreira ; Regina Vincenzi Oliveira
• Associated researchers: Alberto Porta ; Heloisa Sobreiro Selistre de Araújo ; Renato Lajarim Carneiro ; Tiago Venancio
• Associated research grant(s): 23/01626-2 - Study of the integrated profile of cardiovascular autonomic modulation and cardiorespiratory fitness on metabolome and blood count in individuals without diagnosed diseases, AP.R
• Associated scholarship(s): 24/00979-1 - Development of data processing routines and extraction of physiological information from wearable technology., BP.TT; 23/12256-1 - Influence of regular aerobic exercise on blood variables in apparently healthy men, BP.IC; 22/04099-0 - Technical support to the Cardiovascular Physiotherapy Laboratory, BP.TT; + associated scholarships 22/02209-3 - Influence of physical exercise and aging on predictors of aerobic fitness and physical health in apparently healthy men, BP.IC; 21/03432-5 - Analysis of inspiratory muscle oxygenation responses to performing the maximal inspiratory pressure maneuver in patients with chronic obstructive pulmonary disease, BP.IC; 21/02159-3 - Correlation between aerobic fitness and different intensities of activities of daily living by wearable technologies, BP.IC; 20/15769-1 - Technical support to the cardiovascular physiotherapy laboratory in the performance of functional tests in healthy men - part II, BP.TT; 20/13939-7 - Metabolomic analysis by Nuclear Magnetic Resonance (NMR) and mass spectrometry (LC-MS) of blood samples from individuals submitted to cardiorespiratory rehabilitation., BP.PD; 20/01364-0 - Wearables to investigate the relationship between physical activity level, sedentary behavior and aerobic fitness, BP.IC; 19/15040-4 - Omic-based strategies for the investigation of biomarkers for early diagnosis of patients with progressive biliary diseases, BP.DR; 19/19615-1 - Technical support to the laboratory of cardiovascular physiotherapy in the execution of functional tests in healthy men - part I, BP.TT; 19/19474-9 - Technical support to cardiovascular physiotherapy laboratory in functional testing in healthy men - part III, BP.TT; 18/22818-9 - Continuous cardiovascular health evaluation by wearables, BP.DR; 19/10749-5 - Machine learning algorithms for the evaluation of the effect of inspiratory muscle training on the aerobic response in recreational cyclists., BP.PD; 19/10756-1 - Metabolomic analysis by Nuclear Magnetic Resonance of blood samples from individuals submitted to cardiorespiratory rehabilitation., BP.PD; 19/07364-4 - Technical support to the laboratory of cardiovascular physiotherapy in the performance of functional tests in healthy men - part III, BP.TT; 19/03672-6 - Technical support to the laboratory of cardiovascular physiotherapy in the execution of functional tests in healthy men - part I, BP.TT; 19/03669-5 - Technical support to the laboratory of cardiovascular physiotherapy in the execution of functional tests in healthy men - part III, BP.TT; 18/25082-3 - Study of the metabolic profile in active and sedentary aging: metabolomic approach, BP.DD; 18/25519-2 - Analysis of inspiratory muscle oxygenation responses at maximal inspiratory pressure maneuver in healthy men, BP.IC - associated scholarships

Abstract

This project is composed of 3 themes: 1) inspiratory muscle training (IMT), 2) aging and 3) wearables. THEME 1: IMT has brought benefits to several systems of the organism and to the improvement of physical performance. However, there is no consensus on the best intensity of IMT for healthy and physically active individuals, as well as on cardiovascular, respiratory, metabolic, autonomic and metabolic pathway responses. Objectives: to evaluate the effect of IMT using critical inspiratory pressure - PThC (CIP) compared to the 60% group of MIP (MIP60) and Sham (SHAM), in the improvement of physical performance, assessed during the cardiopulmonary exercise test (CPET), as well as cardiorespiratory and metabolic responses, during CPET and inspiratory muscle endurance tests, in recreational cyclists; to identify the activated metabolic pathways, by metabolomic approach, and to evaluate the cardiovascular autonomic modulation at rest before and after IMT. Forty young male recreational cyclists (20-40 years) will be evaluated who will perform: blood collection for metabolomic approach [nuclear magnetic resonance (NMR) and hyphenated liquid chromatography using mass spectrometry (LC-MS)], CPET, PThC determination protocol, cardiovascular autonomic assessment, at rest before and after 11 weeks of IMT. It is expected that after the IMT, the CIP presents cardiovascular, respiratory, autonomic and metabolic demands greater than the MIP60 and SHAM, showing improvement in physical performance; and that the study of the metabolome, allows clarifying the metabolic pathways activated with IMT. THEME 2: The metabolic pathways and cardiovascular oscillations are affected by the aging process. However, there are no studies that compare metabolic changes between healthy active and sedentary individuals, through metabolomics techniques, and their relationship with cardiovascular autonomic modulation. Objectives: to characterize the metabolic changes (study of the metabolome) of active and non-active aging in men aged 20-70 years and their relationship with aerobic capacity and cardiovascular oscillations. One hundred and ten men (55 physically active), apparently healthy, will be evaluated, divided into decades, who will carry out the same evaluations of Theme 1, except the IMT and the determination of PThC. It is expected that the results from the metabolome study will clarify the activated metabolic pathways, as well as the differences between active and non-active aging and their relationship with cardiovascular oscillations. THEME 3: Impairments in cardiorespiratory health (CRH) are related to the onset of chronic noncommunicable diseases (NCDs) and increased mortality. The evaluation of CRH is clinically valuable, but it is done only occasionally and involves the use of medical equipment that is not available to the general population. Thus, there is a need to explore new technologies for the continuous monitoring of CRH by more accessible equipment. Objectives: to use accessible wearable technologies, associated with artificial intelligence (AI) algorithms, for the continuous prediction of CRH in order to identify changes related to the appearance of NCDs. Healthy individuals with risk factors for NCDs and with NCDs will be recruited. The 1st stage will consist of laboratory evaluation of the CRH. The 2nd stage will consist of outpatient vital signs collection, where participants will wear a smart T-shirt with several wearable sensors on board. The 3rd stage consists in the development of AI algorithms that will be trained to predict CRH based on the vital signs collected by the smart shirt. It is expected that the technology developed here will be used throughout physical rehabilitation programs, allowing individual adjustments in real-time and in the effectiveness of such programs; in addition to impacting the prevention of NCDs in the future by early detection of CRH changes. (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ABREU, RAPHAEL MARTINS; CATAI, APARECIDA MARIA; CAIRO, BEATRICE; REHDER-SANTOS, PATRICIA; DA SILVA, CLAUDIO DONISETE; SIGNINI, ETORE DE FAVARI; SAKAGUCHI, CAMILA AKEMI; PORTA, ALBERTO. A Transfer Entropy Approach for the Assessment of the Impact of Inspiratory Muscle Training on the Cardiorespiratory Coupling of Amateur Cyclists. FRONTIERS IN PHYSIOLOGY, v. 11, FEB 25 2020. (17/13402-0, 18/11123-0, 16/22215-7)

BELTRAME, THOMAS; GOIS, MARIANA OLIVEIRA; HOFFMANN, UWE; KOSCHATE, JESSICA; HUGHSON, RICHARD LEE; MORAES FRADE, MARIA CECILIA; LINARES, STEPHANIE NOGUEIRA; TORRES, RICARDO DA SILVA; CATAI, APARECIDA MARIA. Relationship between maximal aerobic power with aerobic fitness as a function of signal-to-noise ratio. Journal of Applied Physiology, v. 129, n. 3, p. 522-532, SEP 2020. (18/19016-8, 16/22215-7, 17/09639-5)

GALDINO, GABRIELA AGUIAR MESQUITA; SILVA, LUIZ EDUARDO VIRGILIO; MOURA-TONELLO, SILVIA CRISTINA GARCIA; MILAN-MATTOS, JULIANA CRISTINA; LINARES, STEPHANIE NOGUEIRA; PORTA, ALBERTO; DA SILVA, THAIS MARQUES; FAZAN JR, RUBENS; BELTRAME, THOMAS; CATAI, APARECIDA MARIA. Heart rate fragmentation is impaired in type 2 diabetes mellitus patients. Diabetes Research and Clinical Practice, v. 196, p. 8-pg., 2022-12-21. (16/22215-7, 10/52070-4, 13/07953-3)

REHDER-SANTOS, PATRICIA; ABREU, RAPHAEL M.; SIGNINI, ETORE DE F.; DA SILVA, CLAUDIO D.; SAKAGUCHI, CAMILA A.; DATO, CARLA C.; CATAI, APARECIDA M.. Moderate- and High-Intensity Inspiratory Muscle Training Equally Improves Inspiratory Muscle Strength and Endurance-A Double-Blind Randomized Controlled Trial. INTERNATIONAL JOURNAL OF SPORTS PHYSIOLOGY AND PERFORMANCE, v. 16, n. 8, p. 1111-1119, AUG 2021. (19/10749-5, 16/22215-7, 19/03669-5)

CAIRO, BEATRICE; DE ABREU, RAPHAEL MARTINS; BARI, VLASTA; GELPI, FRANCESCA; DE MARIA, BEATRICE; REHDER-SANTOS, PATRICIA; SAKAGUCHI, CAMILA AKEMI; DA SILVA, CLAUDIO DONISETE; SIGNINI, ETORE DE FAVARI; CATAI, APARECIDA MARIA; et al. Optimizing phase variability threshold for automated synchrogram analysis of cardiorespiratory interactions in amateur cyclists. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND, v. 379, n. 2212, DEC 13 2021. (16/22215-7, 17/13402-0, 18/11123-0)

DE FAVARI SIGNINI, ETORE; CASTRO, ALEX; REHDER-SANTOS, PATRICIA; CRISTINA MILLAN-MATTOS, JULIANA; MAGALHAES DE OLIVEIRA, JULIANA; MINATEL, VINICIUS; BIANCA FALASCO PANTONI, CAMILA; SOBREIRO SELISTRE DE ARAUJO, HELOISA; FABRIZZI, FERNANDO; PORTA, ALBERTO; et al. Integrative perspective of the healthy aging process considering the metabolome, cardiac autonomic modulation and cardiorespiratory fitness evaluated in age groups. SCIENTIFIC REPORTS, v. 12, n. 1, p. 12-pg., 2022-12-09. (10/52070-4, 20/13939-7, 16/22215-7, 19/15040-4, 20/05965-8, 18/25082-3)

DA SILVA, CLAUDIO D.; DE ABREU, RAPHAEL M.; REHDER-SANTOS, PATRICIA; DE NORONHA, MARCOS; CATAI, APARECIDA M.. Can respiratory muscle training change the blood pressure levels in hypertension? A systematic review with meta-analysis. SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS, v. 31, n. 7, MAR 2021. (16/22215-7)

FRADE, MARIA CECILIA MORAES; BELTRAME, THOMAS; GOIS, MARIANA DE OLIVEIRA; PINTO, ALLAN; TONELLO, SILVIA CRISTINA GARCIA DE MOURA; TORRES, RICARDO DA SILVA; CATAI, APARECIDA MARIA; JAAFAR, ZULKARNAIN. Toward characterizing cardiovascular fitness using machine learning based on unobtrusive data. PLoS One, v. 18, n. 3, p. 18-pg., 2023-03-02. (18/19016-8, 16/22215-7, 17/09639-5, 18/22818-9, 19/16253-1)

DE ABREU, RAPHAEL MARTINS; PORTA, ALBERTO; REHDER-SANTOS, PATRICIA; CAIRO, BEATRICE; SAKAGUCHI, CAMILA AKEMI; DA SILVA, CLAUDIO DONISETE; SIGNINI, ETORE DE FAVARI; MILAN-MATTOS, JULIANA CRISTINA; CATAI, APARECIDA MARIA. Cardiorespiratory coupling strength in athletes and non-athletes. Respiratory Physiology & Neurobiology, v. 305, p. 8-pg., 2022-07-13. (16/22215-7, 18/11123-0, 20/15769-1, 17/13402-0)

REHDER-SANTOS, PATRICIA; MINATEL, VINICIUS; MILAN-MATZOS, JULIANA CRISTINA; SIGNINI, ETORE DE FAVARI; DE ABREU, RAPHAEL MARTINS; DATO, CARLA CRISTINA; CATAI, APARECIDA MARIA. Critical inspiratory pressure - a new methodology for evaluating and training the inspiratory musculature for recreational cyclists: study protocol for a randomized controlled trial. Trials, v. 20, MAY 7 2019. (16/22215-7)

DE ABREU, RAPHAEL MARTINS; PORTA, ALBERTO; REHDER-SANTOS, PATRICIA; CAIRO, BEATRICE; DA SILVA, CLAUDIO DONISETE; SIGNINI, ETORE DE FAVARI; SAKAGUCHI, CAMILA AKEMI; CATAI, APARECIDA MARIA. Effects of inspiratory muscle-training intensity on cardiovascular control in amateur cyclists. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, v. 317, n. 6, p. R891-R902, DEC 2019. (17/13402-0, 18/11123-0, 16/22215-7)

CASTRO, ALEX; SIGNINI, ETORE F.; DE OLIVEIRA, JULIANA MAGALHAES; DI MEDEIROS LEAL, MARIA CAROLINA BEZERRA; REHDER-SANTOS, PATRICIA; MILLAN-MATTOS, JULIANA C.; MINATEL, VINICIUS; PANTONI, CAMILA B. F.; OLIVEIRA, REGINA V.; CATAI, APARECIDA M.; et al. The Aging Process: A Metabolomics Perspective. Molecules, v. 27, n. 24, p. 16-pg., 2022-12-01. (16/22215-7, 19/15040-4, 10/52070-4, 20/05965-8, 20/13939-7, 18/25082-3)

MORAES FRADE, MARIA CECILIA; BELTRAME, THOMAS; VIANA, DAIANE ROBERTA; CARVALHO DA SILVA, MARCELA MARIA; GOIS, MARIANA DE OLIVEIRA; DE FACIO, CARINA ARAUJO; GONZAGA, LUANA APRECIDA; ARCURI, JULIANO FERREIRA; PIRES DI LORENZO, VALERIA AMORIM; CATAI, APARECIDA MARIA. Wearables to monitoring the activities daily living in patients post-hospitalization by COVID-19. European Respiratory Journal, v. 58, p. 3-pg., 2021-09-05. (18/22818-9, 16/22215-7)

CASTRO, A.; SIGNINI, E. F.; REHDER-SANTOS, P.; MILLAN-MATTOS, J. C.; DE OLIVEIRA, J. M.; MINATEL, V.; PANTONI, C. B. F.; OLIVEIRA, R. V.; CATAI, A. M.; FERREIRA, A. G.. The Aging Process: A Metabolomics Perspective. ACTA PHYSIOLOGICA, v. 236, p. 2-pg., 2022-09-01. (20/13939-7, 16/22215-7)

SIGNINI, ETORE; REHDER-SANTOS, PATRICIA; ABREU, RAPHAEL; SAKAGUCHI, CAMILA; SILVA, CLAUDIO; MARCONDES, ANA CAROLINA; RIBEIRO, BRUNO; MINATEL, VINICIUS; MILAN-MATTOS, JULIANA; CARNEIRO, RENATO; et al. Inspiratory muscle metaboreflex during a progressive inspiratory threshold loading test. European Respiratory Journal, v. 58, p. 2-pg., 2021-09-05. (16/14065-5, 16/22215-7, 18/25082-3)

SIGNINI, E. F.; CASTRO, A.; REHDER-SANTOS, P.; MILAN-MATTOS, J. C.; MINATEL, V.; PANTONI, C. B. F.; PORTA, A.; FERREIRA, A. G.; OLIVEIRA, R. V.; CATAI, A. M.. Relationship between serum metabolites and cardiac autonomic modulation in apparently healthy individuals: a metabolomics approach. ACTA PHYSIOLOGICA, v. 236, p. 2-pg., 2022-09-01. (18/25082-3, 10/52070-4, 16/22215-7)