Development of diagnostic test and selection of vaccine candidates for mycoplasmosis in cattle and goats

• Grant number: 17/25686-3
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2019
• End date: July 31, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Jorge Timenetsky
• Grantee: Jorge Timenetsky
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Ana Marcia de Sá Guimarães ; Guilherme Barreto Campos ; Lucas Miranda Marques

Abstract

The intensification of livestock farming has created complex problems in the health of animal production, further challenging the activity of the veterinarian. Despite advances in chemotherapeutic and effective vaccines, diseases caused by microorganisms remain the major obstacle in intensive animal production. Losses from infectious diseases are estimated to be 15-20%. Infections caused by Mollicutes (mycoplasmas) cause a wide range of pathologies associated with genital and respiratory tracts, as well as mammary glands, causing great losses in bovine breeding and goat breeding. Despite progress in studies on therapeutic and prevention methods, strategies still remain inefficient. In this way, the generation of a new alternative that provides better protection against mycoplasma infections is necessary. With this, this project aims at the development of diagnostic tests and the selection of vaccine candidates for mycoplasmosis in cattle and goats. For this, this project will be carried out in three main strands: 1) Test standardization for immunodetection of M. bovis, M. agalactiae and Ureaplasma diversum; 2) Evaluation of the functionality of the immunochromatographic test in an epidemiological study; 3) To analyze the immunogenicity of recombinant proteins of M. bovis, M. agalactiae and U. diversum, for the selection of possible vaccine candidates. The rapid diagnosis of mycoplasmosis under field conditions will allow the rapid decision-making of breeders and producers regarding the actions of discarding or keeping animals in the herds, directly influencing the levels of productivity, reproductive efficiency and competitiveness of the livestock enterprise (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARBOSA, MAYSA SANTOS; MARQUES, LUCAS MIRANDA; TIMENETSKY, JORGE; ROSENGARTEN, RENATE; SPERGSER, JOACHIM; CHOPRA-DEWASTHALY, ROHINI. Host cell interactions of novel antigenic membrane proteins of Mycoplasma agalactiae. BMC Microbiology, v. 22, n. 1, p. 13-pg., 2022-04-08. (17/25686-3, 19/03425-9, 16/23306-6)

SANTOS JUNIOR, MANOEL NERES; SANTOS, RONALDO SILVA; NEVES, WANDERSON SOUZA; FERNANDES, JANAINA MARINHO; DE BRITO GUIMARAES, BRUNA CAROLINA; BARBOSA, MAYSA SANTOS; COELHO SILVA, LUCAS SANTANA; GOMES, CAMILA PACHECO; REZENDE, IZADORA SOUZA; TEIXEIRA OLIVEIRA, CALINE NOVAES; et al. Immunoinformatics and analysis of antigen distribution of Ureaplasma diversum strains isolated from different Brazilian states. BMC Veterinary Research, v. 16, n. 1, OCT 7 2020. (17/25686-3)