How do fuel ethanol yeasts and contaminating lactic acid bacteria respond toward lignocellulosic-derived inhibitors?

• Grant number: 18/17172-2
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2019
• End date: July 31, 2021
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Thiago Olitta Basso
• Grantee: Thiago Olitta Basso
• Host Institution: Escola Politécnica (EP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Ricardo Pinheiro de Souza Oliveira
• Associated research grant(s): 19/08845-6 - Engineering sucrose uptake and phosphorolysis in Escherichia coli, AP.R SPRINT

Abstract

Production of biofuels from lignocellulosic (LC) residues should be fuelling the energy matrix in the near future. Yeasts will play an important role as platform microorganisms for the conversion processes of LC-derived sugars into, for instance, fuel ethanol but also other advanced fuels and chemicals. Fermentation of LC hydrolysates poses many scientific and technological challenges. For example, besides the problem of fermenting pentose sugars, pretreatment processes generate various yeast growth inhibitors (furan-derivatives, phenolics and organic acids), which reduce the efficiency of fermentation. In this context, the present research proposal aims to investigate the effect of LC inhibitors in fuel ethanol yeasts, as well as in contaminating lactic acid bacteria, that normally are present in industrial fermentation processes. This will be accomplished by an in-depth physiological characterization of yeast strains under the presence of inhibitory compounds encountered during industrial fermentation of lignocellulosic-derived feedstock. Complementary to the physiological characterization, yeast strain improvement will be attempted by evolutionary engineering (EE). EE strategies will be attempted using a small-scale sequential batch fermentation setup and/or prolonged chemostat cultivations. Ultimately, we aim to understand how strains of yeasts and contaminating lactic acid bacteria respond to LC-inhibitory compounds and, possible to obtain yeast strains more tolerant toward these compounds, using evolutionary engineering. (AU)

Scientific publications

(16)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

GRACA, CATIA A. L.; DALLE LUCCA, BEATRIZ ARNAUT; COLA, PRISCILA; BASSO, THIAGO O.; TEIXEIRA, ANTONIO CARLOS S. C.. Photodegradation of Enrofloxacin by the Photo-Fenton-Like Reaction Using UVA-Irradiated Iron(III)-Tartrate as a Source of Iron(II). JOURNAL OF ENVIRONMENTAL ENGINEERING, v. 146, n. 12, DEC 1 2020. (18/17172-2, 13/50218-2)

COLA, PRISCILA; PROCOPIO, DIELLE PIEROTTI; ALVES, ADRIANA TABOSA DE CASTRO; CARNEVALLI, LUCIANA REBELO; SAMPAIO, ICARO VIANA; DA COSTA, BRUNO LABATE VALE; BASSO, THIAGO OLITTA. Differential effects of major inhibitory compounds from sugarcane-based lignocellulosic hydrolysates on the physiology of yeast strains and lactic acid bacteria. Biotechnology Letters, v. 42, n. 4, p. 571-582, APR 2020. (18/01759-4, 15/50612-8, 18/17172-2)

ALDER-RANGEL, ALENE; IDNURM, ALEXANDER; BRAND, ALEXANDRA C.; BROWN, ALISTAIR J. P.; GORBUSHINA, ANNA; KELLIHER, CHRISTINA M.; CAMPOS, CLAUDIA B.; LEVIN, DAVID E.; BELL-PEDERSEN, DEBORAH; DADACHOVA, EKATERINA; et al. The Third International Symposium on Fungal Stress - ISFUS. FUNGAL BIOLOGY, v. 124, n. 5, p. 235-252, MAY 2020. (15/24305-0, 16/11386-5, 18/17172-2, 17/22669-0, 13/50518-6, 18/20571-6, 10/06374-1, 16/14542-8)

GIACON, THAMIRIS GUERRA; DE GOIS E CUNHA, GABRIEL CAETANO; ELIODORIO, KEVY PONTES; DE SOUZA OLIVEIRA, RICARDO PINHEIRO; BASSO, THIAGO OLITTA. Homo- and heterofermentative lactobacilli are distinctly affected by furanic compounds. Biotechnology Letters, v. 44, n. 12, p. 15-pg., 2022-12-01. (19/13826-0, 18/17172-2)

ELIODORIO, KEVY PONTES; PENNACCHI, CESARE; CUNHA, GABRIEL CAETANO DE GOIS E; MORANDIM-GIANNETTI, ANDREIA DE ARAUJO; GIUDICI, REINALDO; BASSO, THIAGO OLITTA. Effects of caramelization and Maillard reaction products on the physiology of Saccharomyces cerevisiae. FUNGAL BIOLOGY, v. 127, n. 12, p. 10-pg., 2023-12-13. (21/13614-3, 19/08393-8, 21/13701-3, 18/17172-2)

DE ARAUJO, THIAGO MACHADO; DA CUNHA, MARCEL MENEZES LYRA; BARGA, MARCELO CALIDE; DELLA-BIANCA, BIANCA ELI; BASSO, THIAGO OLITTA. Production of flavor-active compounds and physiological impacts in immobilized Saccharomyces spp. cells during beer fermentation. Letters in Applied Microbiology, v. 77, n. 9, p. 10-pg., 2024-09-16. (18/08288-7, 18/17172-2)

BASSO, THIAGO O.; VENTURINI, ANDRESSA M.; CECCATO-ANTONINI, SANDRA REGINA; GOMBERT, ANDREAS K.. Microbial ecology applied to fuel ethanol production from sugarcane. FEMS MICROBIOLOGY ECOLOGY, v. 101, n. 11, p. 12-pg., 2025-11-01. (18/17172-2, 22/03293-8, 22/15256-0, 22/03052-0)

GIACON, THAMIRIS GUERRA; VILELA, NATHALIA; TRIVELLIN, CECILIA; BASSO, THIAGO OLITTA; OLSSON, LISBETH. Lignocellulosic hydrolysate composition influences contamination profiles in ethanol production. Bioresource Technology, v. 435, p. 11-pg., 2025-11-01. (23/07553-7, 18/17172-2, 22/03052-0)

PROCOPIO, DIELLE PIEROTTI; LEE, JAE WON; SHIN, JONGHYEOK; TRAMONTINA, ROBSON; AVILA, PATRICIA FELIX; BRENELLI, LIVIA BEATRIZ; SQUINA, FABIO MARCIO; DAMASIO, ANDRE; RABELO, SARITA CANDIDA; GOLDBECK, ROSANA; et al. Metabolic engineering of Saccharomyces cerevisiae for second-generation ethanol production from xylo-oligosaccharides and acetate. SCIENTIFIC REPORTS, v. 13, n. 1, p. 14-pg., 2023-11-06. (17/15477-8, 20/05784-3, 15/50612-8, 18/17172-2, 18/01759-4, 22/08958-8, 21/04254-3, 15/50590-4, 22/05731-2, 19/18075-3)

DE OLIVEIRA LINO, FELIPE SENNE; GARG, SHILPA; LI, SIMONE S.; MISIAKOU, MARIA-ANNA; KANG, KANG; VALE DA COSTA, BRUNO LABATE; BEYER-PEDERSEN, TOBIAS SVEND-AAGE; GIACON, THAMIRIS GUERRA; BASSO, THIAGO OLITTA; PANAGIOTOU, GIANNI; et al. Strain dynamics of contaminating bacteria modulate the yield of ethanol biorefineries. NATURE COMMUNICATIONS, v. 15, n. 1, p. 13-pg., 2024-06-22. (15/50684-9, 18/17172-2)

BERMEJO, PAMELA MAGALI; BADINO, ALBERTO; ZAMBERLAN, LUCIANO; RAGHAVENDRAN, VIJAYENDRAN; BASSO, THIAGO OLITTA; GOMBERT, ANDREAS KAROLY. Ethanol yield calculations in biorefineries COMMENT. FEMS Yeast Research, v. 21, n. 8, p. 6-pg., 2021-12-13. (18/17172-2, 18/11405-5)

PRADO, CLEITON D.; MANDRUJANO, GUSTAVO P. L.; SOUZA, JONAS. P.; SGOBBI, FLAVIA B.; NOVAES, HOSANA R.; DA SILVA, JOAO P. M. O.; ALVES, MATEUS H. R.; ELIODORIO, KEVY P.; CUNHA, GABRIEL C. G.; GIUDICI, REINALDO; et al. Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation. BIOTECHNOLOGY FOR BIOFUELS, v. 13, n. 1, OCT 27 2020. (16/10130-7, 18/17172-2, 19/08393-8, 18/20697-0)

VALE DA COSTA, BRUNO LABATE; RAGHAVENDRAN, VIJAYENDRAN; MERCIER FRANCO, LUIS FERNANDO; CHAVES FILHO, ADRIANO DE BRITTO; YOSHINAGA, MARCOS YUKIO; MIYAMOTO, SAYURI; BASSO, THIAGO OLITTA; GOMBERT, ANDREAS KAROLY. Forever panting and forever growing: physiology of Saccharomyces cerevisiae at extremely low oxygen availability in the absence of ergosterol and unsaturated fatty acids. FEMS Yeast Research, v. 19, n. 6, SEP 2019. (15/14109-0, 18/17172-2)

ELIODORIO, KEVY PONTES; CUNHA, GABRIEL CAETANO DE GOIS E.; WHITE, BRIANNA A.; PATEL, DEMISHA H. M.; ZHANG, FANGYI; HETTEMA, EWALD H.; BASSO, THIAGO OLITTA; GOMBERT, ANDREAS KAROLY; RAGHAVENDRAN, VIJAYENDRAN. Blocking Mitophagy Does Not Significantly Improve Fuel Ethanol Production in Bioethanol Yeast Saccharomyces cerevisiae. Applied and Environmental Microbiology, v. 88, n. 5, p. 12-pg., 2022-03-01. (17/08464-7, 19/08393-8, 18/17172-2)

WALKER, GRAEME M.; BASSO, THIAGO O.. Mitigating stress in industrial yeasts. FUNGAL BIOLOGY, v. 124, n. 5, p. 387-397, MAY 2020. (18/17172-2, 18/20571-6)

ELIODORIO, KEVY PONTES; DE GOIS E CUNHAA, GABRIEL CAETANO; MUELLER, CAROLINE; LUCARONI, ANA CAROLINA; GIUDICI, REINALDO; WALKER, GRAEME MAXWELL; ALVES, SERGIO LUIZ, JR.; BASSO, THIAGO OLITTA; GADD, GM; SARIASLANI, S. Advances in yeast alcoholic fermentations for the production of bioethanol, beer and wine. ADVANCES IN APPLIED MICROBIOLOGY, VOL 109, v. 109, p. 59-pg., 2019-01-01. (15/50684-9, 19/08393-8, 18/17172-2)