| Grant number: | 18/14468-8 |
| Support Opportunities: | Regular Research Grants |
| Start date: | May 01, 2019 |
| End date: | October 31, 2021 |
| Field of knowledge: | Health Sciences - Medicine |
| Principal Investigator: | Vinicius de Lima Vazquez |
| Grantee: | Vinicius de Lima Vazquez |
| Host Institution: | Hospital do Câncer de Barretos. Barretos , SP, Brazil |
| City of the host institution: | Barretos |
| Associated researchers: | Ana Carolina Laus ; Rui Manuel Vieira Reis |
| Associated scholarship(s): | 20/00176-5 - Platform - liquid biopsy for melanomas,
BP.TT 19/13390-8 - PLATFORM: liquid biopsy for melanomas, BP.TT |
Abstract
Melanoma accounts for more than 90% of skin cancer deaths. BRAF, NRAS and TERT promoter mutations, together, affect more than two thirds of the melanomas. Screening for BRAF mutations in tumor tissue is important for therapeutic decision and prediction of response to target therapy. Monitoring of metastatic disease using this marker necessitates the availability of tumor sample. Liquid biopsy is a promising method of detecting DNA fragments in body fluids. Implementing a minimally invasive method in a rapidly progressing disease could decrease the number of diagnostic exams, identify patients with neoplastic progression and select a population that would benefit from targeted therapy. The objective of this study is to standardize the Digital PCR technique (dPCR) and to determine the sensitivity and specificity of the ctDNA (circulating DNA) technique for the detection of specific mutations (BRAF / NRAS / TERT) in plasma samples from patients with melanoma within a scenario of clinical practice. This is a prospective and cross-sectional study, in which patients with advanced melanoma with mutation of at least one of the genes of interest in the tissue will be selected previously the treatment, for evaluation of the mutations in the plasma and will be monitored quantitatively over time for the plasma mutation detection according to the evolution of the disease. In conclusion, the results of this study may establish a simplified method of monitoring the presence of metastatic disease for melanoma patients, for becoming a powerful diagnostic tool. (AU)
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