A lipidomics study applied to Rectal Cancer: biochemical-molecular mechanisms and assessment of the response to neoadjuvant therapy

• Grant number: 18/13317-6
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2020
• End date: January 31, 2023
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Patrícia de Oliveira Carvalho
• Grantee: Patrícia de Oliveira Carvalho
• Host Institution: Universidade São Francisco (USF). Campus Bragança Paulista. Bragança Paulista , SP, Brazil
• Associated scholarship(s): 20/11461-2 - Multivariate platforms for lipidomic study in biological samples, BP.TT

Abstract

The current approach to advanced rectal adenocarcinoma involves total excision of the mesorectum associated to neoadjuvant chemo-radiotherapy. Patient response to therapy varies and knowledge of the mechanisms involved in the response is incomplete. Metabolic modifications occurring in carcinogenesis demonstrate a relationship with treatment evolution and the response to it and the latter influence the tumor micro-environment. Continuing the studies undertaken by our group, this project sets out to study the biochemical-molecular bases underlying alterations to patient's plasma lipidomic profiles and to evaluate the effect of the response to neoadjuvant therapy by examining the molecular signatures of the plasma of patients resistant to treatment. Chromatography and mass spectrometry will be used to characterize the lipids and biochemical and molecular measurements (ELISA and Western Blot) to quantify the oxidation products and the expressions of enzymes associated to lipid metabolism. The lipidomics study is expected to collaborate towards a better understanding of rectal cancer physiopathology, relating it to a characteristic signature capable of identifying both the presence of rectal cancer and its behavior in response neoadjuvant therapy. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, ANNA MARIA A. P.; MESSIAS, MARCIA C. F.; DUARTE, GUSTAVO H. B.; DE SANTIS, GABRIELLE K. D.; MECATTI, GIOVANA C.; PORCARI, ANDREIA M.; MURGU, MICHAEL; SIMIONATO, ANA VALERIA C.; ROCHA, THALITA; MARTINEZ, CARLOS A. R.; et al. Plasma Lipid Profile Reveals Plasmalogens as Potential Biomarkers for Colon Cancer Screening. METABOLITES, v. 10, n. 6, JUN 2020. (17/25017-4, 18/13317-6)

SANCHES, PEDRO H. G.; DE OLIVEIRA, DANILO C.; DOS REIS, IVAN G. M.; FERNANDES, ANNA M. A. P.; SILVA, ALEX A. R.; EBERLIN, MARCOS N.; CARVALHO, PATRICIA O.; DUARTE, GUSTAVO H. B.; PORCARI, ANDREIA M.. Fitting Structure-Data Files (.SDF) Libraries to Progenesis QI Identification Searches. Journal of the Brazilian Chemical Society, v. N/A, p. 7-pg., 2023-02-03. (18/13317-6, 19/04314-6)

CONTESINI, FABIANO JARES; DAVANCO, MARCELO GOMES; BORIIN, GUSTAVO PAGOTTO; VANEGAS, KATHERINA GARCIA; GONCALVES CIRINO, JOAO PEDRO; DE MELO, RICARDO RODRIGUES; MORTENSEN, UFFE HASBRO; HILDEN, KRISTIINA; CAMPOS, DANIEL ROSSI; CARVALHO, PATRICIA DE OLIVEIRA. Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry. CATALYSTS, v. 10, n. 9, p. 33-pg., 2020-09-01. (18/13317-6, 17/14253-9)

SANCHEZ-VINCES, SALVADOR; DUARTE, GUSTAVO HENRIQUE BUENO; MESSIAS, MARCIA CRISTINA FERNANDES; GATINONI, CAROLINE FERNANDA ALVES; SILVA, ALEX AP. ROSINI; SANCHES, PEDRO HENRIQUE GODOY; MARTINEZ, CARLOS AUGUSTO REAL; PORCARI, ANDREIA M.; CARVALHO, PATRICIA DE OLIVEIRA. Rectal Cancer Tissue Lipidome Differs According to Response to Neoadjuvant Therapy. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 14, p. 12-pg., 2023-07-01. (18/13317-6)