Role of tumor necrosis factor-alfa in pulpal and periapical inflammation and repair

• Grant number: 19/00204-1
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2019
• End date: August 31, 2021
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Francisco Wanderley Garcia de Paula e Silva
• Grantee: Francisco Wanderley Garcia de Paula e Silva
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• City of the host institution: Ribeirão Preto

Abstract

Tumor necrosis factor-z (TNF-z) role in in vitro induction of dental pulp cell proliferation, migration and differentiation of dental pulp cells is already known and indicates that this mediator might be and important regulator of the recruitment of cells to injured sites for tissue regeneration or repair. Nevertheless, all these phenomena were demonstrated in vitro in cell culture models, opening avenues for in vivo investigation. Therefore, the general goal of this research is to investigate the role of TNF-z in dental pulp inflammation and repair and in the development of apical periodontitis, using genetically deficient mice to TNF-z receptor-1 (TNFRSF1) in comparison to wild type animals (C57Bl6). A pulp capping model will be used to evaluate the dental pulp response after tissue exposure and protection. To evaluate the periapical bone response, a model will be used in which the pulp tissue will be exposed to the buccal environment for microbial contamination. The specific objectives will be to investigate: (i) the degree of tissue inflammation by quantifying inflammatory cells and evaluating gene and protein expression, (ii) microbial contamination in pulp and periapical tissues, (iii) reabsorption of mineralized tissues by computerized microtomography and microscopic analysis, and (iv) osteoclastogenesis on bone tissue and dental root surfaces. In addition, TNF-z estimulated dental pulp cells will be used to dissect the possible mechanisms involved in the regulation of proteins important for the mineralization process under pro-inflammatory stimuli. (AU)

Scientific publications

(7)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

LÉA ASSED BEZERRA DA SILVA; LÍDIA REGINA DA COSTA HIDALGO; MANOEL DAMIÃO DE SOUSA-NETO; MAYA FERNANDA MANFRIN ARNEZ; FREDERIC BARNETT; PATRICIA MARÍA GATÓN HERNÁNDEZ; LÚCIA HELENA FACCIOLI; FRANCISCO WANDERLEY GARCIA PAULA-SILVA. Cytotoxicity and Inflammatory Mediators Release by Macrophages Exposed to Real Seal XT and Sealapex Xpress. Brazilian Dental Journal, v. 32, n. 1, p. 48-52, 2021-04-02. (19/00204-1)

LAMARQUE, GIULIANA DE CAMPOS CHAVES; LEME, ROBERTA DUARTE; ALMEIDA JUNIOR, LUCIANO APARECIDO DE; BOMBONATO-PRADO, KARINA FITTIPALDI; SEGATO, RAQUEL ASSED BEZERRA; GEORGE, ANNE; PAULA-SILVA, FRANCISCO WANDERLEY GARCIA. Effects triggered by tumor necrosis factor-α in immortalized murine dental pulp and pre-osteoblastic cells. BRAZILIAN ORAL RESEARCH, v. 39, p. 11-pg., 2025-01-01. (19/00204-1, 20/06607-8)

SALES, LARISSA STHEFANI; SILVA-SOUSA, ALICE CORREA; NASCIMENTO, GLAUCE CRIVELARO; DEL BEL, ELAINE; PAULA-SILVA, FRANCISCO WANDERLEY GARCIA. Effects of cannabidiol on biomineralization and inflammatory mediators expression in immortalized murine dental pulp cells and macrophages under pro-inflammatory conditions. Journal of Dentistry, v. 153, p. 7-pg., 2024-12-24. (19/00204-1)

SORGI, CARLOS ARTERIO; DE CAMPOS CHAVES LAMARQUE, GIULIANA; VERRI, MARAISA P.; NELSON-FILHO, PAULO; FACCIOLI, LUCIA HELENA; PAULA-SILVA, FRANCISCO WANDERLEY GARCIA. Multifaceted effect of caffeic acid against Streptococcus mutans infection: microbicidal and immunomodulatory agent in macrophages. Archives of Microbiology, v. 203, n. 6, p. 2979-2987, AUG 2021. (19/00204-1)

TAIRA, THAISE MAYUMI; RIBEIRO, VITOR LUIS; SILVA RIBEIRO, YURI JIVAGO; BEZERRA DA SILVA, RAQUEL ASSED; BEZERRA DA SILVA, LEA ASSED; LUCISANO POLITI, MARILIA PACIFICO; FACCIOLI, LUCIA HELENA; GARCIA PAULA-SILVAA, FRANCISCO WANDERLEY. Gene expression data of inflammatory mediators in apical periodontitis in 129 (wild type) and 5-lipoxygenase knockout mice. DATA IN BRIEF, v. 40, p. 8-pg., 2022-02-01. (19/00204-1, 10/17611-4)

LORENCETTI-SILVA, FRANCINE; SALES, LARISSA STHEFANI; LAMARQUE, GIULIANA DE CAMPOS CHAVES; CAIXETA, GIOVANA AMORIM; ARNEZ, MAYA FERNANDA MANFRIN; FACCIOLI, LUCIA HELENA; PAULA-SILVA, FRANCISCO WANDERLEY GARCIA. Effects of inflammation in dental pulp cell differentiation and reparative response. FRONTIERS IN DENTAL MEDICINE, v. 3, p. 8-pg., 2022-08-02. (14/07125-6, 19/00204-1)

ALMEIDA-JUNIOR, LUCIANO APARECIDO; DE CARVALHO, MARCIO SANTOS; ALMEIDA, LANA KEI YAMAMOTO; SILVA-SOUSA, ALICE CORREA; SOUSA-NETO, MANOEL DAMIAO; SILVA, RAQUEL ASSED BEZERRA; SILVA, LEA ASSED BEZERRA; PAULA-SILVA, FRANCISCO WANDERLEY GARCIA. TNF-a-TNFR1 Signaling Mediates Inflammation and Bone Resorption in Apical Periodontitis. JOURNAL OF ENDODONTICS, v. 49, n. 10, p. 12-pg., 2023-09-28. (19/02432-1, 19/00204-1)