Capillary electrophoresis as analytical technique for the determination of biogenic amines in food matrices

• Grant number: 19/03582-7
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2019
• End date: January 31, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Elisabete Alves Pereira
• Grantee: Elisabete Alves Pereira
• Host Institution: Centro de Ciências e Tecnologias para a Sustentabilidade (CCTS). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil
• Associated researchers: Luís Francisco Moreira Gonçalves

Abstract

Contaminated and/or deteriorated foods cause numerous losses in the food industry, besides the consumption of this type of food brings several risks for the health. People have been concerned about food safety issues and, for that matter, there are various indicators of food quality, such as the amount of biogenic amines. Biogenic amines may be present in a variety of foods and beverages, such as dairy products, fish, meat and fermented beverages or vegetables. Their harmful effects may cause various symptoms related to food poisoning. There is no Brazilian legislation for the most diverse structures present in various foods (only for histamine in the muscle of some fresh fish species), for this reason the development of methodologies for the analysis of these substances is very important both analytically and toxicologically. The present project aims to develop and optimize new analytical methods and then apply them in the identification and quantification of the main biogenic amines in different food matrices (cheese, yoghurt and other products) using capillary electrophoresis as analytical technique, evaluating the profile of these compounds in commercial products from the Brazilian market. For the derivatization step, different reagents including dansyl chloride (DNS-Cl), 4-methoxybenzenesulfonyl fluoride (MOBS-F) and benzoyl chloride (Ben-Cl) will be evaluated. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES MANTOANELLI, JESSICA OLIVEIRA; GONCALVES, LUIS MOREIRA; PEREIRA, ELISABETE ALVES. Dansyl Chloride as a Derivatizing Agent for the Analysis of Biogenic Amines by CZE-UV. Chromatographia, v. 83, n. 6, APR 2020. (18/14425-7, 19/03582-7)

DONEGATTI, TIAGO AUGUSTO; LOBATO, ALNILAN; DUEK, ELIANA A. R.; GONCALVES, LUIS MOREIRA; PEREIRA, ELISABETE ALVES. Derivatization-free determination of aminoglycosides by CZE-UV in pharmaceutical formulations. ELECTROPHORESIS, v. 41, n. 18-19, AUG 2020. (18/14425-7, 19/03582-7, 19/11214-8)