Point-of-care (POC) tests for simultaneous or individual detection of enteroxygenic Escherichia coli (ETEC) and E. coli Shiga toxin (STEC)

• Grant number: 17/25210-9
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: June 01, 2019
• End date: November 30, 2021
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Agreement: Secretary of State Government, State of São Paulo
• Principal Investigator: Thiago Pinotti Segato
• Grantee: Thiago Pinotti Segato
• Company: Partecurae Pesquisa e Desenvolvimento Ltda
• CNAE: Fabricação de produtos farmoquímicos ; Pesquisa e desenvolvimento experimental em ciências físicas e naturais
• City: São Carlos
• Pesquisadores principais: Beatriz Cutilak Bianchi ; Regiane de Fátima Travensolo
• Associated researchers: Ana Maria Moro ; André Nogueira Castro de Barros ; Emanuel Carrilho ; Jeanne Blanco de Molfetta ; Letícia Barboza Rocha ; RODRIGO LEMOS LOVATO ; Roxane Maria Fontes Piazza
• Associated scholarship(s): 19/11723-0 - Point-Of-Care (POC) tests for simultaneous or individual detection of Enterigoxygenic Escherichia coli (ETC) and E. coli Shiga toxin (STEC), BP.PIPE

Abstract

Diarrheal disease is responsible for major global public health problems, causing about 1.2 million annual deaths in children under five years of age. In Brazil, mainly in the north and northeast, this disease is still one of the most important causes of infant mortality. Among the causative pathogens such as diarrheogenic Escherichia coli are responsible for 30 to 40% of episodes of acute diarrhea in developing countries. Among diarrheogenic E. coli, EPEC, ETEC, EAEC and STEC are the most epidemiological significance related to most diarrheal outbreaks. With the exception of EAEC, a composition not yet described, is a complete list of all categories, all like others cited above, as one or more characteristic virulence factor, which allows its diagnosis. In this context, the diagnosis is an important one to minimize and control occurrences. Antibodies are important tools in the detection of several pathogens, monoclonal antibodies, presented high specificity and immune sera obtained in communications bodies. An application of specific antibodies without the development of an immunochromatographic (IC) test, or POC, allowed to detect ETEC and STEC through their toxins and to demonstrate pathway treatment, to enable their future use in clinical laboratories and hospitals. For this, the refinement of performance parameters, stability and scaling studies and resources for subsequent scale production of the IC test. (AU)