Evaluation of probiotic orange juice in induced colitis in murine model

• Grant number: 18/14658-1
• Support Opportunities: Regular Research Grants
• Duration: May 01, 2019 - October 31, 2021
• Field of knowledge: Health Sciences - Nutrition
• Principal Investigator: Lizziane Kretli Winkelströter Eller
• Grantee: Lizziane Kretli Winkelströter Eller
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil
• Associated researchers: Sandra Cristina Genaro ; Wilson Romero Nakagaki
• Associated scholarship(s): 20/12040-0 - Animal experimentation and laboratory parameters for analysis of induced colitis, BP.TT

Abstract

Probiotics have become increasingly popular due to the beneficial effects on human health as prevention and / or treatment of certain clinical situations, such as infection by Helicobacter pylori, inflammatory bowel disease, diarrhea, cardiovascular diseases, allergies, among others. Several probiotics have been used in food products, mainly fermented dairy foods, however, there has been increased interest in non-dairy probiotics because of the growing number of vegans and the largest number of individuals with dietary restrictions. Chronic diseases, such as inflammatory bowel diseases, are considered one of the great problems of the modern population, since they tend to be progressive, generating important repercussions on the quality of life of its patients, which cause changes in the social, psychological and professional spheres. Another factor, which exacerbates the situation of patients with inflammatory bowel diseases in Brazil, is the lack of studies and dissemination of this group of pathologies, which contributes to delayed diagnosis and increased morbidity. In this context, the importance of studies with application of new probiotic products in the beneficial action for the prevention and treatment of diseases is highlighted. This work aims to evaluate the effects of probiotic orange juice on induced colitis in an animal model. Orange juice isolates with probiotic potential will be used to produce probiotic orange juice. The quality of the juice will be evaluated by fermentation parameters such as growth of the microorganism, pH and viability by standard plate counting. In the animal experiment male Wistar rats will be used and induction of colitis will be performed with sodium dextran sulfate (4%). Then the effects generated with the use of fermented orange juice and the drug sulfasalazine will be observed. Macroscopic and histological evaluation will be performed to evaluate the severity of tissue damage and the inflammatory process through the evaluation of cytokines and inflammatory markers. In addition, the composition of the intestinal microbiota will be evaluated by real-time PCR. The results will be compiled into graphs and tables; the statistical analysis will be performed considering a level of significance of 0.05. The use of probiotics in the treatment and prevention of diseases is a very current topic and presents itself as a very interesting therapeutic possibility since probiotics can be useful tools in the promotion of human health. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA VIEIRA, KAROLINNY CRISTINY; FERREIRA, CAMILA DA SILVA; TOSO BUENO, EMILI BRUNA; DE MORAES, YURI ALLISSON; CAMPAGNOLO GONCALVES TOLEDO, ANA CLARA; NAKAGAKI, WILSON ROMERO; PEREIRA, VALERIA CATANELI; WINKELSTROTER, LIZZIANE KRETLI. Development and viability of probiotic orange juice supplemented by Pediococcus acidilactici CE51. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 130, AUG 2020. (18/14658-1)