| Grant number: | 19/11816-8 |
| Support Opportunities: | Regular Research Grants |
| Start date: | August 01, 2019 |
| End date: | January 31, 2022 |
| Field of knowledge: | Agronomical Sciences - Food Science and Technology - Food Science |
| Principal Investigator: | Joao Paulo Fabi |
| Grantee: | Joao Paulo Fabi |
| Host Institution: | Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| City of the host institution: | São Paulo |
| Associated scholarship(s): | 21/08531-1 - EFFECT OF MODIFIED FOOD FIBERS FROM PAPAYAS USING IN VIVO MODEL OF RATS WITH CHEMICALLY INDUCED COLON CARCINOGENESIS,
BP.TT 20/02628-0 - EFFECT OF MODIFIED FOOD FIBERS FROM PAPAYAS USING IN VIVO MODEL OF RATS WITH CHEMICALLY INDUCED COLON CARCINOGENESIS, BP.TT 19/25323-3 - Effect of modified fibers of papayas in three-dimensional colon cancer human cell culture, BP.TT |
Abstract
The consumption of dietary fibers (DF), especially the non-digestible polysaccharides, has been related to the decrease in the incidence of colon cancer. Fruits, such as papayas, are foods rich in pectins, which constitute a large amount of the DF fraction of the fruit. Papaya has a very fast ripening, with consequent pulp softening and modification of the DF structures. The DF from ripe papayas have been linked to a number of beneficial biological effects in a structurally-dependent manner, including decreased colon (2D) cell proliferation in colon cancer cells, and decreased pre-neoplastic lesions in the colon of rats chemically induced for cancer development. However, the variation of DF composition in papayas is large, and previous studies have shown that the chemical modification of green papaya DF decreased and uniformized the molecular weight of pectins, increasing its biological effects such as decreased cell proliferation in 2D culture of colon cancer cells. Thus, the present project proposal aims to evaluate the effects of modified DF of unripe and ripe papayas, especially pectins, in 3D co-culture of spheroids using colorectal cancer cells (HCT116 and HT29) and human fibroblasts (CCD- 18Co), and to verify the effect of the consumption of the modified DF of unripe and ripe papayas in rats with pre-neoplastic colon lesions. In this way, it will be possible to evaluate whether the structure-dependent effects of modified DF, especially pectins, remain in a more complex model than the bi-dimensional cell culture. In this model, it will also be possible to evaluate the metabolism of AF consumed by the rat intestinal microbiota and the production of metabolites such as short chain fatty acids (SCFA). From a health and technological point of view, the results obtained from the present proposal could expand the knowledge of how the structural alterations of DF impact in the development of colon cancer by expanding the creation of new food supplements that may have preventive action in the development of colon cancer and that can also be used as adjuvants to the chemotherapy treatment of colon cancer. (AU)
| Articles published in Agência FAPESP Newsletter about the research grant: |
| More itemsLess items |
| TITULO |
| Articles published in other media outlets ( ): |
| More itemsLess items |
| VEICULO: TITULO (DATA) |
| VEICULO: TITULO (DATA) |