Advanced search
Start date
Betweenand

Screening of placental genomic imprinting processes on DLK1-DIO3 locus in hypothyroid patients: involvement of T3 in offspring's DM2 susceptibility

Abstract

The thyroid hormone is widely involved in metabolic control processes, from fetal development to adulthood. Environmental disruptors in the gestational period, which could affect epigenetic control-ling processes, may predispose the susceptibility to metabolic diseases in adult life, like diabetes mellitus type 2. Although many studies have been associated with a reduction in Dio3 mRNA expression to altered DNA methylation status on imprinted DLK1-DIO3 locus with the occurrence of glucose intolerance, the exact mechanisms have not been fully elucidated. Thus, our hypothesis it's that hypothyroidism may be considered a disruptive factor in placental imprinting mechanisms, conferring susceptibility to the development of T2DM. Therefore, the aim of this study is to compare the gene expression profile and the methylation status of DLK1-DIO3 locus in placentas from hypothyroid patients. To do this, 70 patients will be recruited, which will subdivided in 2 groups of hypothyroidism and healthy pregnant and we will carry out the comparative study of the methylation profile of placental DNA by pyrosequenciation (Pyromark Q24) after bisulfite conversion of IG- DMR and MEG3 -DMR in addition to the expression profile of mRNA by qRT-PCR. The comparison between proportions among groups will be calculated with Chi-Square Test, the minimum significative difference between groups will be 40%, with 80% power and significance of 1%. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.