NOVA1 RNAi silencing in glioblastoma cell lines: proteomic analysis of the targets...
| Grant number: | 19/10868-4 |
| Support type: | Regular Research Grants |
| Duration: | November 01, 2019 - October 31, 2021 |
| Field of knowledge: | Biological Sciences - Morphology |
| Principal Investigator: | Luciana Amaral Haddad |
| Grantee: | Luciana Amaral Haddad |
| Home Institution: | Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Assoc. researchers: | Carla Columbano de Oliveira ; Luis Eduardo Soares Netto ; Lygia da Veiga Pereira |
Abstract
Fragile mental retardation protein (FMRP), encoded by the FMR1 gene, has been detected in pre- and postsynaptic neuronal processes. It plays central roles in translational repression of several dendritic mRNAs, which have translation activated upon synaptic stimulation, notably by glutamate. Other DNA- and RNA-mediated roles have been assigned to FMRP. Different FMRP isoforms have been proposed due to FMR1 transcript alternative splicing and protein electrophoretic gel mobility variations. However, a limited number of specific functional roles have been experimentally demonstrated for FMRP isoforms. In this proposal, we aim to understand the generation of functional diversity by FMR1 alternative splicing. (AU)