Evaluation of PTEN inhibition and compartmentalization effects in the presence of neuroinflammation: participation of glutamatergic signaling

• Grant number: 19/12974-6
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2020
• End date: July 31, 2022
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Elisa Mitiko Kawamoto Iwashe
• Grantee: Elisa Mitiko Kawamoto Iwashe
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Cristoforo Scavone

Abstract

Although originally described as tumor suppressor, PTEN (phospatase and tensin homolog deleted on chromosome ten), has been studied by researches related to cellular processes such as cell growing, cell proliferation, cell survival, metabolism and cell migration, with a potential modulatory role on neurogenesis, synaptic plasticity and, more recently, inflammation. The location of PTEN inside the cell can be both cytosolic and nuclear as its functions seem to depend on where PTEN is located within the cell. This project will focus on PTEN role about neuroinflammation and it has as objetive to evaluate the possible effects of PTEN compartmentalization (nucleus/cytosol) and PTEN inhibition in the presence of inflammation induced by lypopolysacharide (LPS) in primary glia and mixed primary glia and neuronal culture. The better understanding of PTEN involvement on signaling pathways direct or indirectly involved on neuroinflammation could lead to the development of new therapeutic strategies for more effective treatments against diseases associated with PTEN deletion or mutation, neuroinflammation and neurodegenerative diseases. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KAWAMOTO, E. M.; CARARO-LOPES, M. M.; KINOSHITA, P. F.; QUINTAS, L. E. M.; LIMA, L. S.; ANDREOTTI, D. Z.; SCAVONE, C.. Influence of Nitric Oxide-Cyclic GMP and Oxidative STRESS on Amyloid-beta Peptide Induced Decrease of Na,K-ATPase Activity in Rat Hippocampal Slices. Journal of Membrane Biology, v. 254, n. 5-6, SI, JUL 2021. (16/07427-8, 16/22996-9, 18/14289-6, 19/12974-6, 14/14199-6)

NAGY, GABRIELA SPINA; KAWAMOTO, ELISA MITIKO; BRIDI, JESSIKA CRISTINA. The role of PTEN signaling in synaptic function: Implications in autism spectrum disorder. Neuroscience Letters, v. 759, AUG 10 2021. (20/08005-5, 16/07427-8, 19/12974-6, 19/10044-1)

VASCONCELOS, ANDREA R.; DA PAIXAO, AMANDA G.; KINOSHITA, PAULA F.; ORELLANA, ANA M.; SCAVONE, CRISTOFORO; KAWAMOTO, ELISA M.. Toll-like Receptor 4 Signaling is Critical for the Adaptive Cellular Stress Response Effects Induced by Intermittent Fasting in the Mouse Brain. Neuroscience, v. 465, p. 142-153, JUN 15 2021. (14/01435-3, 19/12974-6, 16/22996-9, 16/07376-4, 06/57729-9, 16/07427-8, 11/12255-8, 09/11915-4)

KINOSHITA, PAULA FERNANDA; MARQUES ORELLANA, ANA MARIA; NAKAO, VINICIUS WATANABE; DE SOUZA PORT'S, NATACHA MEDEIROS; MENEZES QUINTAS, LUIS EDUARDO; KAWAMOTO, ELISA MITIKO; SCAVONE, CRISTOFORO. The Janus face of ouabain in Na+/K+-ATPase and calcium signalling in neurons. British Journal of Pharmacology, MAR 2021. (16/22996-9, 19/12974-6, 16/07427-8, 18/14289-6)

KINOSHITA, PAULA FERNANDA; MARQUES ORELLANA, ANA MARIA; NAKAO, VINICIUS WATANABE; DE SOUZA PORT'S, NATACHA MEDEIROS; MENEZES QUINTAS, LUIS EDUARDO; KAWAMOTO, ELISA MITIKO; SCAVONE, CRISTOFORO. The Janus face of ouabain in Na+/K+-ATPase and calcium signalling in neurons. British Journal of Pharmacology, v. 179, n. 8, p. 13-pg., 2021-03-30. (16/22996-9, 18/14289-6, 16/07427-8, 19/12974-6)