Research Grants 19/24950-4 - Endocrinologia, Imunidade - BV FAPESP
Advanced search
Start date
Betweenand

Temporal dynamics of the inflammatory response in amphibians

Abstract

In recent decades, there has been a steep decline in amphibian populations around the globe. This decline is associated with multiple causes acting in a synergistic way, highlighting the increase in the prevalence and outbreaks of emerging diseases, the loss, fragmentation and disconnection of habitats, environmental pollution and climate change in multiple scales. In this context of exposure of amphibians to multiple stressors and pathogens, it is imperative a better understanding of the endogenous defense processes in this group and their modulation by stress response mediators. Considering these mediators, we highlight glucocorticoids and melatonin, hormones that play a prominent role in immunomodulatory activity. The objective of this project is to evaluate the temporal dynamics of changes in hormone secretion and gene expression of cytokines that participate in the assembly and regulation of the inflammatory response in anurans following an immune challenge. Our hypothesis is that, during three successive phases of the dynamics of the inflammatory process, we will first observe an increase in the production of pro-inflammatory cytokines, activation of the hypothalamic-pituitary-interrenal axis and inhibition of the central secretion of melatonin. The second phase should be characterized by an increase in the expression of regulatory cytokines. Finally, the third phase should be characterized by reduced hypothalamic-pituitary-interrenal axis activity, resumption of central melatonin secretion, and reduced expression of proinflammatory and regulatory cytokines. To test this hypothesis, immunological challenges will be performed with lipopolysaccharide (LPS), mimicking a bacterial infection in toads (Rhinella diptycha) and bullfrogs (Rana catesbeiana). We will follow the dynamics of hormone secretion (corticosterone and melatonin) in the circulation, expression of the enzymes of the melatonin production pathway in the brain and eyes, and the production of different cytokines in the spleen in response to this immunological challenge, by using RNAseq to analyzes transcriptome data and identify genes differentially expressed in response to LPS. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TITON, STEFANNY C. M.; TITON JR, BRAZ; MUXEL, SANDRA M.; DE FIGUEIREDO, AYMAM C.; FLORESTE, FELIPE R.; LIMA, ALAN S.; GOMES, FERNANDO R.; ASSIS, VANIA R.. Day vs. night variation in the LPS effects on toad's immunity and endocrine mediators. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 267, p. 9-pg., . (16/01782-0, 15/23801-4, 19/24950-4, 14/16320-7)
FLORESTE, FELIPE R.; TITON JR, BRAZ; TITON, STEFANNY C. M.; MUXEL, SANDRA M.; GOMES, FERNANDO R.; ASSIS, VANIA R.. Time Course of Splenic Cytokine mRNA and Hormones during a Lipopolysaccharide-Induced Inflammation in Toads. INTEGRATIVE AND COMPARATIVE BIOLOGY, v. N/A, p. 11-pg., . (15/23801-4, 14/16320-7, 16/01782-0, 19/24950-4, 20/00370-6)
TITON, STEFANNY CHRISTIE MONTEIRO; TITON JUNIOR, BRAZ; DE FIGUEIREDO, AYMAM COBO; FLORESTE, FELIPE RANGEL; FLORESTE, ALAN SIQUEIRA; CYRINO, JOAO CUNHA; GOMES, FERNANDO RIBEIRO. Plasma steroids and immune measures vary with restraint duration in a toad (Rhinella icterica). General and Comparative Endocrinology, v. 318, p. 9-pg., . (14/16320-7, 19/24950-4)
TITON, STEFANNY CHRISTIE MONTEIRO; JUNIOR, BRAZ TITON; ASSIS, VANIA REGINA; DE FIGUEIREDO, AYMAM COBO; FLORESTE, FELIPE RANGEL; LIMA, ALAN SIQUEIRA; GOMES, FERNANDO RIBEIRO. Testosterone immunomodulation in free-living and captive Rhinella icterica male toads. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, v. 378, n. 1882, p. 10-pg., . (14/16320-7, 19/24950-4, 16/01782-0)
GOMES, FERNANDO RIBEIRO; MADELAIRE, CARLA BONETTI; MORETTI, EDUARDO HERMOGENES; MONTEIRO TITON, STEFANNY CHRISTIE; ASSIS, VANIA REGINA. Immunoendocrinology and Ecoimmunology in Brazilian Anurans. INTEGRATIVE AND COMPARATIVE BIOLOGY, v. N/A, p. 17-pg., . (15/23801-4, 20/09399-7, 11/03030-2, 14/16320-7, 19/24950-4, 18/00406-0)

Please report errors in scientific publications list using this form.
X

Report errors in this page


Error details: