Effect of artificial saliva substitutes and fluoride application on the development of root caries under a microcosm biofilm model simulating patients subjected to head and neck radiation

• Grant number: 19/21797-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2020
• End date: July 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Ana Carolina Magalhães
• Grantee: Ana Carolina Magalhães
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated researchers: Marília Afonso Rabelo Buzalaf

Abstract

Radiation-related root dental caries is one of the adverse effects found in patients submitted to a head and neck radiation therapy. The first objective of this work will be to verify the influence of biofilm from irradiated patient and tooth that has undergone radiation on the development of root caries as well as to test new formulations of artificial saliva with respect to the prevention of root caries under these situations in vitro (step 1). The second objective will be to define the best fluoride agent for preventing root caries comparing different products intended for professional application in vitro. For the step 1, 240 bovine root dentin samples will be divided into subgroups according to: 1) irradiated (70 Gy) dentin or not; 2) the type of human biofilm used for the formation of microcosm biofilm (biofilm from irradiated and non-irradiated patient) and 3) type of artificial saliva used for the treatment of this patient (6 formulations, highlighting the experimental ones containing sugarcane cystatin and hemoglobin, only for the condition irradiated tooth and biofilm from irradiated patient). For the step 2, 180 irradiated dentin samples will be treated with one of the fluoride agents (4% TiF4 varnish, 5.42% NaF varnish, placebo varnish, 30% SDF solution and no treatment) and submitted to microcosm biofilm formation from biofilm of irradiated patients. The microcosm biofilm will be formed for 5 days, using a McBain saliva with 0.2% of sucrose at 37ºC and 5% CO2. The response variables will be: bacteria viability and extracellular polysaccharide biovolume by fluorescence using a specifics kits and confocal microscope (steps 1 and 2); CFU counting for total microorganisms, total Streptococci, Streptococcus mutans, total lactobacilli and yeast (steps 1 and 2) and quantification of lactic acid production using lactate dehydrogenase kit and microplate reader (step 2). The demineralization will be quantified by transverse microradiography (steps 1 and 2). The data will be submitted to statistical analysis (p <0.05). (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, BEATRIZ MARTINES; SILVA, MAYARA SOUZA; BRAGA, ALINE SILVA; KERGES BUENO, PATRICIA SANCHES; DA SILVA SANTOS, PAULO SERGIO; RABELO BUZALAF, MARILIA AFONSO; MAGALHAES, ANA CAROLINA. Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro. SCIENTIFIC REPORTS, v. 11, n. 1, MAR 16 2021. (19/21797-0, 19/07241-0)

FRANCESE, MONIQUE MALTA; BOLZAN GONCALVES, ISABELA VIEIRA; VERTUAN, MARIELE; DE SOUZA, BEATRIZ MARTINES; MAGALHAES, ANA CAROLINA. The protective effect of the experimental TiF4 and chitosan toothpaste on erosive tooth wear in vitro. SCIENTIFIC REPORTS, v. 12, n. 1, p. 7-pg., 2022-04-30. (19/21797-0, 18/26369-4)

ALINE SILVA BRAGA; KIM RAFAELA RICCI; ANA CAROLINA MAGALHÃES. Effect of anaerobic or/and microaerophilic atmosphere on microcosm biofilm formation and tooth demineralization. Journal of Applied Oral Science, v. 31, 2023-06-02. (19/01730-9, 17/17249-2, 19/21797-0)

OLIVEIRA VENTURA, TALITA MENDES; SANTOS, KARINA OLIVEIRA; BRAGA, ALINE SILVA; GRIZZO THOMASSIAN, LARISSA TERCILIA; RABELO BUZALAF, MARILIA AFONSO; BARBIERI, FABIO AUGUSTO; KALVA-FILHO, CARLOS AUGUSTO; FARIA, MURILO HENRIQUE; MAGALHAES, ANA CAROLINA. Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results. SPORT SCIENCES FOR HEALTH, v. 18, n. 3, p. 15-pg., 2022-01-10. (19/21797-0, 20/02175-6)

VENTURA, TALITA MENDES OLIVEIRA; SANTOS, KARINA OLIVEIRA; BRAGA, ALINE SILVA; THOMASSIAN, LARISSA TERCILIA GRIZZO; BUZALAF, MARILIA AFONSO RABELO; KALVA-FILHO, CARLOS AUGUSTO; FARIA, MURILO HENRIQUE; BARBIERI, FABIO AUGUSTO; MAGALHAES, ANA CAROLINA. Proteomic profile of saliva in patients with Parkinson's disease after the practice of interval exercise. PARKINSONISM & RELATED DISORDERS, v. 98, p. 2-pg., 2022-05-01. (20/02175-6, 19/21797-0)

SANTOS, KARINA OLIVEIRA; FILHO, DALTON MULLER PESSOA; VENTURA, TALITA MENDES OLIVEIRA; THOMASSIAN, LARISSA TERCILIA GRIZZO; MACEDO, ANDERSON GEREMIAS; BUZALAF, MARILIA AFONSO RABELO; BRAGA, ALINE SILVA; FARIA, MURILO HENRIQUE; MAGALHAES, ANA CAROLINA. Salivary proteomic profile of response to different resistance training protocols: A case report. Cell Biochemistry and Function, v. 42, n. 1, p. 11-pg., 2024-01-01. (20/02175-6, 19/21797-0)

DE SOUZA, BEATRIZ MARTINES; FRANCISCO, EDUARDO LISTA; BRAGA, ALINE SILVA; SANTOS, PAULO SERGIO DA SILVA; BUZALAF, MARILIA AFONSO RABELO; MAGALHAES, ANA CAROLINA. In vitro effect of TiF₄/NaF solution on the development of radiation-induced dentin caries. Journal of Applied Oral Science, v. 32, p. 7-pg., 2024-01-01. (19/07241-0, 19/21797-0)

BRAGA, ALINE SILVA; ABDELBARY, MOHAMED MOSTAFA HEFNY; KIM, RAFAELA RICCI; DE SOUZA ROSA DE MELO, FERNANDA PEREIRA; SALDANHA, LUIZ LEONARDO; DOKKEDAL, ANNE LIGIA; CONRADS, GEORG; ESTEVES-OLIVEIRA, MARCELLA; MAGALHAES, ANA CAROLINA. The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development. ANTIBIOTICS-BASEL, v. 11, n. 3, p. 14-pg., 2022-03-01. (17/00556-0, 17/17249-2, 19/21797-0, 18/26506-1)

VERTUAN, MARIELE; DA SILVA, JULIA FRANCA; DE OLIVEIRA, ANA CLARA MOTA; DA SILVA, THAYNA TEODORO; JUSTO, ANDREZA PERES; ZORDAN, FERNANDA LAUDARES SILVA; MAGALHAES, ANA CAROLINA. The in vitro Effect of Dentifrices With Activated Charcoal on Eroded Teeth. INTERNATIONAL DENTAL JOURNAL, v. 73, n. 4, p. 6-pg., 2023-08-01. (21/06143-4, 19/21797-0)

DE SOUZA, BEATRIZ MARTINES; BRAGA, ALINE SILVA; VERTUAN, MARIELE; SASSAKI, SUSAN; ARAUJO, TAMARA TEODORO; SANTOS, PAULO SERGIO DA SILVA; BUZALAF, MARILIA AFONSO RABELO; MAGALHAES, ANA CAROLINA. Influence of irradiated dentin, biofilm and different artificial saliva formulations on root dentin demineralization. HELIYON, v. 10, n. 16, p. 10-pg., 2024-08-30. (19/07241-0, 19/21797-0)