Exploiting STED microscopy to unveil cellular damage induced by photosensitizing molecules

• Grant number: 19/23223-1
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2022
• End date: October 31, 2023
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Agreement: Lund University
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Pedro Henrique Benites Aoki
• Grantee: Pedro Henrique Benites Aoki
• Principal researcher abroad: Christelle Prinz
• Institution abroad: Lund University, Lund, Sweden
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
• Associated researchers: Carlos José Leopoldo Constantino ; Diogo Volpati ; Elke Hebisch ; Priscila Alessio Constantino
• Associated research grant: 18/16713-0 - Photodynamic therapy: from molecular effects in model systems toward photodynamic efficiency in cell culture, AP.R

Abstract

This one-year SPRINT project is focused in a research area that is emerging rapidly and expanding the limits to resolve cell structures and their functions; stimulated emission depletion (STED) microscopy of living cells tagged to photodynamic therapy. By bridging the groups from Lund University (SE) and UNESP (Brazil) we aim to approach the underlying mechanisms of cell-photosensitizer (PS) interaction in photodynamic therapy (PDT) of cancer cells using super-resolution microscopy, with potential expansion to other areas. PDT is minimally invasive procedure that induces cell death by photooxidative reactions. Simplified models of tumor cells have already been photosensitized in the ongoing project of the Brazilian group, however, the precise cellular localization of the PS in cancer cells remains unknown. The present project will tackle this issue using the super-resolution STED microscope available at Lund University. With the aid of the STED microscope, we shall determine the precise end point (organelles) of the PS after cellular uptake, which will generate knowledge to further impact the pathway of cellular death triggered by illumination. In a series of meeting between the two groups, we have already identified possible experimental setups, materials and cell lines that would be well suited to perform this initial yet attractive project. This mobility project grant will catalyze and expand considerably the interactions between researchers at Lund University and UNESP, developing further our collaboration, and solidifying a foundation for more extensive research partnership. (AU)